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82,530 results

Showing: 9461 - 9480 of 82530 results

  1. pCMV-hA3A-eBE-Y130F

    Plasmid
    #113423
    Purpose
    Expresses hA3A-eBE-Y130F in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    hAPOBEC3A_Y130F
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113423-plasmid-map-sequence-id-217432

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  4. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Publication
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61549-plasmid-map-sequence-id-212455

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  7. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Publication
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107455-plasmid-map-sequence-id-207549

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  9. pmCherry-C1-mTrim21ΔRING-Box

    Plasmid
    #105517
    Purpose
    Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells
    Depositor
    Melina Schuh
    Publication
    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRIM21 (Trim21 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of RING domain (aa124-462)
    Promoter
    CMV
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105517-plasmid-map-sequence-id-204988

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  11. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid
    #105802
    Purpose
    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-mRuby3; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105802-plasmid-map-sequence-id-203396

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  13. pCAGGS_3E5E_luciferase

    Plasmid
    #103055
    Purpose
    Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Publication
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ebolavirus minigenome
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Dec. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    103055-plasmid-map-sequence-id-447476

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  15. pC0041-RanCas13b crRNA backbone

    Plasmid
    #103852
    Purpose
    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-RanCas13b DR-BbsI-BbsI-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103852-plasmid-map-sequence-id-199153

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  16. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100087-plasmid-map-sequence-id-197556

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  17. pCLIPf-H2B Control Plasmid

    Plasmid
    #101134
    Purpose
    Expression of CLIP-tagged Histone H2B in mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Histone H2B (H2BC11 Human)
    Tags
    CLIP-tag (CLIPf)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101134-plasmid-map-sequence-id-194159

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  18. pSNAP-CaaX Control Plasmid

    Plasmid
    #101133
    Purpose
    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaaX Box
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101133-plasmid-map-sequence-id-194147

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  20. pLenti-Mito-Car-GECO

    Plasmid
    #100765
    Purpose
    Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Joseph Gordon
    Publication
    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1
    Insert
    Lenti-Mito-Car-GECO
    Use
    Lentiviral
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100765-plasmid-map-sequence-id-193070

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Showing: 9461 - 9480 of 82530 results