78,128 results

1. gTBEv3

   Plasmid

   #220619

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   hUNG2-nSpCas9
   

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-FKBP-MTM1

   Plasmid

   #51614

   Purpose

   Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   FKBP
   MTM1 (MTM1 Human, 1812)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

   Plasmid

   #106309

   Purpose

   The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
   

   Tags

   6xflag-3xHaloTag(MtoL)

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-p53

   Plasmid

   #22725

   Depositor

   Shinya Yamanaka

   Article

   Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALB-bio-His

   Plasmid

   #52176

   Purpose

   Expresses full-length Serum albumin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ALB (ALB Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

   Plasmid

   #162333

   Purpose

   Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSpCas9-2xVP64
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1 Flag Raptor

   Plasmid

   #26633

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-TCF1

   Plasmid

   #40620

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   TCF7 (TCF7 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-MinE-EGFP_IRES-Blast

    Plasmid

    #213113

    Purpose

    Expression of MinE-EGFP in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB30 (hANGPTL8-V5-His)

    Plasmid

    #109114

    Purpose

    Expresses V5 and His-tagged human ANGPTL8 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (ANGPTL8 Human)
    

    Tags

    6x His and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLVX-TetOne-Puro-mitoLbNOX-FLAG

    Plasmid

    #218667

    Purpose

    pLVX-TetOne-Puro-mitoLbNOX-FLAG

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoLbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-pmExRai-AKAR2

    Plasmid

    #161755

    Purpose

    Plasma membrane-targeted ExRai-AKAR2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    Lyn-ExRai-AKAR2
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-Ef1a-InteinC-CreC

    Plasmid

    #187615

    Purpose

    AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    InteinC-CreC
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-RIPK1

    Plasmid

    #78834

    Purpose

    To overexpress RIPK1 in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI H2B-RFP

    Plasmid

    #92398

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B RFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. peGFP C3-SPG20-PAAA

    Plasmid

    #218581

    Purpose

    Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Proline 172 to Alanine and Tyrosine 174 t…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. peGFP C1-SPG20 SC domain

    Plasmid

    #218580

    Purpose

    Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-417

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PRS_8-eGFP

    Plasmid

    #192589

    Purpose

    AAV-vector for fluorescent labeling of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    PRSX8

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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