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p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSC

Plasmid

#46896

Purpose

expression of scrambled shRNA

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

scrambled

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-Arl13b-mCardinal

Plasmid

#194542

Purpose

Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Arl13b (Arl13b Mouse)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLJC2-MTHFD1-3xFLAG

Plasmid

#217429

Purpose

Lentiviral overexpression of human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

MTHFD1 (MTHFD1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAB2076 pAAV REPAIR.t1

Plasmid

#176323

Purpose

pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Inserts

human codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site

Use

AAV and CRISPR

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

E488Q, only the deaminase domain (aa 276-702 are …

Promoter

EFS (short EF1alpha) and hU6

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHA-eIF4E

Plasmid

#17343

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

eIF4E (EIF4E Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV Ptdss2-V5

Plasmid

#175143

Purpose

Lentiviral expression of mouse Ptdss2-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Ptdss2 (Ptdss2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCRPVsheLL

Plasmid

#46952

Purpose

encode CRPV L1 and L2, Bicistronic, too large to self-package

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

CRPV L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-hMAPT

Plasmid

#231583

Purpose

Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

Depositor

Chaolin Zhang

Article

Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

Insert

human MAPT (MAPT Human)

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/Envelope

Plasmid

#120821

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

Envelope ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSYFP2-C1

Plasmid

#22878

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCDH-IFNA-neo

Plasmid

#225710

Purpose

The pCDH-IFNA-neo plasmid is designed for the overexpression of human IFNα in mammalian cells. This plasmid was utilized to engineer A549 cells for stable IFNα overexpression.

Depositor

Eun Hee Han

Article

Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

Insert

IFNA (IFNA1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

CREB (Creb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

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dCas12Max-CBE

Plasmid

#195337

Purpose

vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized dCas12Max-CBE

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMIEG3-c-Jun

Plasmid

#40348

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

c-Jun (JUN Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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ER-CanlonicSF

Plasmid

#178343

Purpose

To explore endoplasmic reticulum lactate dynamics

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

ER-CanlonicSF

Tags

ER Retention Signal, ER Signal Exon 1, and ER Sig…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-hXRCC1-mCherry-Hygro

Plasmid

#176532

Purpose

mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

X-Ray Repair Cross Complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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