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79,340 results

Showing: 11701 - 11720 of 79340 results

  5. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  6. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  14. pKAM-BFP-Puro

    Plasmid
    #101864
    Purpose
    Lentiviral empty backbone, dual BFP and puro markers
    Depositor
    Archibald Perkins
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    101864-plasmid-map-sequence-id-211112

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  17. pD4sE_SC12

    Plasmid
    #219721
    Purpose
    DV4 stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV4 sE SC12
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219721-plasmid-map-sequence-id-433923

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  18. pD1sE_SC12

    Plasmid
    #219718
    Purpose
    DV1 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV1 sE SC12
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…
    Available Since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    219718-plasmid-map-sequence-id-433425

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  20. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Article
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available Since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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Showing: 11701 - 11720 of 79340 results