78,292 results

1. pHiUGE smFP-V5 donor (all-in-one) ORF2

   Plasmid

   #200388

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Nopt

   Plasmid

   #231704

   Purpose

   Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
   

   Use

   Vsv expression

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HA-Nrxn1beta AS4(-)

   Plasmid

   #59409

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hPDGFRA-GFP

   Plasmid

   #22919

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-ClpX

   Plasmid

   #234733

   Purpose

   Human-codon optimized E. coli ClpX in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ClpX
   

   Expression

   Mammalian

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ExRai-AktAR2

   Plasmid

   #184047

   Purpose

   Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   ExRai-AktAR2
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 CDK7 HA (P#633)

   Plasmid

   #14647

   Depositor

   Matija Peterlin

   Article

   Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

   Insert

   Cdk7 (CDK7 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2904

   Plasmid

   #143388

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   DACH1 (DACH1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. HyPer7.2DAAO-NES

    Plasmid

    #168301

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmScarlet_Giantin_C1

    Plasmid

    #85048

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. cyto-Ruby3-iATPSnFR1.0

    Plasmid

    #102551

    Purpose

    expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    Ruby3-iATPSnFR1.0
    

    Tags

    Ruby3

    Expression

    Mammalian

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmStayGold_I3

    Plasmid

    #239336

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Integrin β3-GFP

    Plasmid

    #205091

    Purpose

    Express GFP-tagged human integrin β3 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 3 (ITGB3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-GFP

    Plasmid

    #11154

    Purpose

    Mammalian expression vector for expression of GFP (EF1a promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EF1 alpha promoter (EEF1A1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO.Nfib.Hygro

    Plasmid

    #117271

    Purpose

    Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Nfib (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    IRES and hygromycin resistant cassette (C termina…

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-Klf4

    Plasmid

    #13370

    Purpose

    Retroviral expression of mouse Klf4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Kruppel-like factor 4 (gut) (Klf4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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