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pMU103

Plasmid

#73934

Purpose

This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.

Depositor

Zhanyuan Zhang

Article

Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d

Insert

bar and GUS cassettes

Expression

Plant

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN2

Plasmid

#96977

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN1

Plasmid

#96976

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEG302 5aa SunTag VP64 g4+g17 (FWA)

Plasmid

#119672

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0112

Plasmid

#117548

Purpose

Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PABE-2

Plasmid

#115623

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCO489

Plasmid

#139732

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_AtuMas:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

AtuMAS

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0040

Plasmid

#117607

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB221

Plasmid

#68612

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB330

Plasmid

#68658

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB319

Plasmid

#68647

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [2_n-1] (GB1206)

Plasmid

#75407

Purpose

tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [2_n-1]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE3

Plasmid

#79442

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE64

Plasmid

#79446

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE1

Plasmid

#79440

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pZA71

Plasmid

#174785

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker

Tags

3xFLAG

Expression

Plant

Mutation

WT

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pZA68

Plasmid

#174781

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E/D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA66

Plasmid

#174779

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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