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Plasmid#136133PurposePlasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygRDepositorInsertp5-35S:HygR p5-MpUBE2:mTurq-N7
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJRA104
Plasmid#209337PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEE515
Plasmid#149288PurposeT-DNA encoding TRV2 complete genome with MCSDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1_CsR-Ck1
Plasmid#136124PurposeL1 in position 1 (next to LB in L2 construct), for CsRDepositorInsertp5-35Sx2:mALS_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGG-TurboID-3XNLS
Plasmid#209392PurposeTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAP4
Plasmid#136079PurposeAcceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.DepositorInsert
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ230 (LbCpf1)
Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE687
Plasmid#167299PurposeVector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB433
Plasmid#74824PurposeGateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCk4_spacer
Plasmid#136074PurposeUse when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDESTpK7WG2mito
Plasmid#158726PurposePlant mitoTALEN vector assembly, Destination vectorDepositorInsertmitochondrial presequence in front of attR1 in pk7WG2
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-B
Plasmid#198234PurposepPLTP::BBM cassette in pBUE411DepositorInsertpPLTP::BBM cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterPLTPAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKb-TnpB1
Plasmid#215333PurposeISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI siteDepositorInsertRice codon optimized ISDra2 TnpB
UseTagsNot applicableExpressionPlantMutationPromoterRice ubiquitin promoter for TnpB and OsU3 promote…Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3TERM_Nos-35S
Plasmid#136116Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_Nos-35S (A. tumefaciens, CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk3_spacer
Plasmid#136073PurposeUse when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19/NLS-mChe
Plasmid#183162PurposeConstruct for transient expression of cell nucleus-targeted mCherry in plants.DepositorInsertNLS-mCherry
UseTags16 aa nuclear localization signal and 16 aa nucl…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSup_35Senh(fwd)_35Spr+synJ
Plasmid#149422PurposePlasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.DepositorInsert35S core enhancer
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only