-
Plasmid#170914PurposeGoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpEstradiol_InducibleAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#104780PurposepSC218UG-At Ubiquitin10:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTX171
Plasmid#89258PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM165/166-48nt
Plasmid#84091Purposetarget miRNA165/166 for destruction in Arabidopsis thalianaDepositorInsertSTTM165/166
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGGG-AH-VRT-A2
Plasmid#163703PurposeWheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)DepositorInsertsOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
UseTagsExpressionPlantMutationPromoterRice - Os Actin promoter and Triticum polonicum V…Available sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRETtv-2in1-NC
Plasmid#105122PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmTRQ2 and mVenusExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Rg
Plasmid#61654PurposeGateway vector for generating red fluorescent protein fusions in fungiDepositorInsertmonomeric red fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-EXOB-11
Plasmid#197688PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEXOB enhancer-blocking insulator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-STU
Plasmid#138110PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminatorDepositorInsertLbCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 nog
Plasmid#115480PurposeCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISV-CRISPR/Cas9PGmUbi
Plasmid#209189PurposeExpression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selectionDepositorInsertsCas9
DsRed(deltaB)
UseTagsNLSExpressionPlantMutationmissing BsaI site and plant-codon optimized with …PromoterCaMV 35S and GmUbi from Glycine max (L.) Merr. cv…Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:BFP:T35S (GB3416)
Plasmid#160637PurposeTU for the constitutive expression of Blue Fluorescent ProteinDepositorInsertBFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGE670
Plasmid#153236PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE393
Plasmid#149283PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW505 - pOp4:3xGFP-NLS
Plasmid#116005Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR
UseTagslinker-NLSExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB405
Plasmid#68670PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-NC
Plasmid#105121PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmCherry and mEGFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT0
Plasmid#210548PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT0
Plasmid#210551PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporterDepositorInsertpU6::AAVS1gRNA::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT1
Plasmid#210552PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporterDepositorInsertpU6::AAVS1gRNA::LT1::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT2
Plasmid#210553PurposeBinary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporterDepositorInsertpU6::AAVS1gRNA::LT2::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum atpB/accD
Plasmid#123582PurposeMoChlo kitDepositorInsertN. tabacum atpB/accD integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDW1775
Plasmid#13456DepositorTypeEmpty backboneUseTagsAcV5ExpressionPlantMutationPromoterAvailable sinceDec. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
lvl0 alcSynth-34S
Plasmid#153411PurposeEthanol system inducible promoterDepositorInsertalcSynth-34S promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_222
Plasmid#91116PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A2.0
Plasmid#99896PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-GPXC71S
Plasmid#191698PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677DepositorInsertredox(ro)GFP2-GPXC71S
UseTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMV-U
Plasmid#112797PurposeUniversal BeYDV-derived vector for cloning of up to four gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB103-Citrine
Plasmid#79137PurposeBinary vectors for expression of Citrine fluorescent protein in M. polymorphaDepositorInsertCitrine
UseTagsCitrineExpressionPlantMutationPromoterMpEF1a promoterAvailable sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCJJ345
Plasmid#117504PurposeYAO:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtYAOAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only