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pH7WG2B-OsMIR390-B/c

Plasmid

#61465

Purpose

Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

OsUBI

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTW2065

Plasmid

#236574

Purpose

ISYmu1 PDS3 gRNA2 TRV2 Architecture_A

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_Hyg

Plasmid

#159701

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mCherry

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19/Vc-C

Plasmid

#183158

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB105

Plasmid

#68559

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB123

Plasmid

#68577

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd6-PBE

Plasmid

#204858

Purpose

For cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM65881

Plasmid

#153215

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB518

Plasmid

#74860

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTC223

Plasmid

#70019

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-TurboID

Plasmid

#231293

Purpose

For transiently expressing fusion proteins with N terminal TurboID for proximity labeling in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-mCitrine

Plasmid

#231281

Purpose

For stably expressing fusion proteins with C terminally tagged mCitrine in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mEGFP

Plasmid

#231287

Purpose

For transiently expressing fusion proteins with N terminally tagged mEGFP in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mCitrine

Plasmid

#231289

Purpose

For transiently expressing fusion proteins with N terminally tagged mCitrine in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mScarletI

Plasmid

#231290

Purpose

For transiently expressing fusion proteins with N terminally tagged mScarletI in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mCitrine

Plasmid

#231297

Purpose

For transiently expressing fusion proteins with C terminally tagged mCitrine in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mScarletI

Plasmid

#231298

Purpose

For transiently expressing fusion proteins with C terminally tagged mScarletI in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-Vn

Plasmid

#183153

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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