4,438 results

1. 35S-GWY-VP16/pAM

   Plasmid

   #201232

   Purpose

   Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GWY-VP16
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH47742::p35S-PE2max-NC::tEURb7

   Plasmid

   #227693

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSL.R.Ly

   Plasmid

   #227697

   Purpose

   Reengineered geminiviral replicon vector

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ203 (pMDC32-Ubi1)

   Plasmid

   #86207

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Expression

   Plant

   Promoter

   Maize ubiquitin 1 (ZmUbi1)

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. UBQ:RUBY

   Plasmid

   #160909

   Purpose

   RUBY under the control of Ubiquitin promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and UBIQUITIN promoter
   

   Expression

   Plant

   Promoter

   UBIQUITIN

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH47742:: pRPS5a-PE2max-NC::tHSP

   Plasmid

   #227694

   Purpose

   Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   PE2max-NC being driven by pRPS5a and terminated by tHSP
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   pRPS5a

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p16 nlsGPS1

   Plasmid

   #101081

   Purpose

   GA biosensor expression plasmid

   Depositor

   Wolf Frommer, Alexander Jones

   Article

   Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

   Insert

   nlsGPS1
   

   Tags

   cMyc

   Expression

   Plant

   Promoter

   p16 (AT3G60245)

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-dzCas9-Act3.0

    Plasmid

    #158414

    Purpose

    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHW101020

    Plasmid

    #222454

    Purpose

    Level 1, Position 2 containing 35S::CUP2-p65::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    35S::CUP2-p65::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIRECT_22A

    Plasmid

    #91133

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ131B2.0

    Plasmid

    #99885

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ132B2.0

    Plasmid

    #99888

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pATUBR

    Plasmid

    #245488

    Purpose

    Binary Plasmid for plant transformation expressing RUBY from the Arabidopsis thaliana polyubiquitin 10 gene promoter and the terminator from the pea RBCS gene. Kanamycin plant selection

    Depositor

    Beth Rowan

    Insert

    pAtUBI-RUBY-tRBCS
    

    Expression

    Plant

    Promoter

    Polyubiquitin 10 gene from Arabidopsis thaliana

    Available Since

    Nov. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT47

    Plasmid

    #223419

    Purpose

    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pM-CBE

    Plasmid

    #196293

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding CBE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. nlsSalicS1

    Plasmid

    #232190

    Purpose

    Novel FRET-based biosensor for salicylic acid

    Depositor

    Alexander Jones

    Article

    Tang et al Science. 2025 Oct 9;390(6769):188-194. d

    Insert

    nlsSalicS1
    

    Tags

    His-tag

    Expression

    Plant

    Promoter

    p16

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpRY-PCE(cm24Cre)

    Plasmid

    #242426

    Purpose

    for chromosome editing

    Depositor

    Caixia Gao

    Article

    Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

    Insert

    SpRY-PE, cm24Cre
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ141D2.0

    Plasmid

    #99906

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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