4,625 results
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Plasmid#191784PurposeSpCas9 expression in plantsDepositorInsertAtUbi10::Cas9 +13 introns-T-E9
UseCRISPRExpressionPlantAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10
Plasmid#197965PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspco-nCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterAtU6-26 and pUBQ10Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S_OMEGA(pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM55351
Plasmid#169092PurposeMoClo level 1 35S promoter-BvADHα-Nos terminator transcription unitDepositorInsert35S promoter-BvADHα-Nos terminator
UseMoclo cloning vectorExpressionPlantAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTC208
Plasmid#70017PurposeTALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl VirusDepositorInsertNuclease (TALENs) + Donor + GVR
UseTALENExpressionPlantAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVR
Plasmid#138117PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoterDepositorInsertMb2Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V and N573RAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIGS3.1 (FF537)
Plasmid#35249DepositorTypeEmpty backboneExpressionPlantPromoterCaMV 35SAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-TBS-11
Plasmid#197689PurposePhytobrick (MoClo) Level 0 PartDepositorInsertTBS enhancer-blocking insulator
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-CmYLCV-4
Plasmid#197694PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCmYLCV promoter
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2-AA012
Plasmid#185751PurposeVector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
Tagsmembrane localization tag Lti6bExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NC
Plasmid#105123PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmVenus and tagRFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-CN
Plasmid#105120PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmVenus and tagRFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
L1P79
Plasmid#195940PurposeLevel 1 pGATA23 reporter : pGATA23-mScarlet-NLS-tRBCSDepositorInsertpGATA23-mScarlet-NLS-tRBCS
ExpressionPlantAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
Plasmid#192427PurposeTo act as the on state for testing of the alt Vika recombinase.DepositorInsertAct2::vox-OCS-vox::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192432PurposeTo act as a 2-input state of the NOR gate.DepositorInsertAct2::FRT-B3RT-Rluc-B3RT-FRT
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH80
Plasmid#128191PurposeLevel1 ZmUBIp::xCas9 3.7:NOStDepositorInsertZmUBIp::xCas9 3.7:NOSt
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:EDLL:tNos (GB1190)
Plasmid#75404PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional ActivatorDepositorInsertdCas:EDLL
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLXMA L2A
Plasmid#153360PurposeExpression vector for plants with medium-copy number origin of replicationDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-As
Plasmid#138094PurposeGolden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CERK1 D441V-YFPc-1
Plasmid#102403Purposesplit YFP. Plant expression of CERK1 D441V-YFPc-1DepositorAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only