1,779 results

1. pCZGY405

   Plasmid

   #172694

   Purpose

   Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   rab-3 (rab-3 Nematode)
   

   Tags

   Punc-25 and mcherry

   Expression

   Worm

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD283

   Plasmid

   #66824

   Purpose

   YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   YPET-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized YPET

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Phsp-16 cz::caspase-3 (p17) [TU#823]

   Plasmid

   #16086

   Depositor

   Martin Chalfie

   Article

   Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

   Insert

   cz :: caspase-3 (p17) (CASP3 Human)
   

   Expression

   Worm

   Available Since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW6.01

   Plasmid

   #62882

   Purpose

   Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   GFP::MEG-3
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   meg-3

   Available Since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK2020

   Plasmid

   #171932

   Purpose

   express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

   Depositor

   Judith Kimble

   Article

   Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

   Insert

   Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
   

   Tags

   superfolder GFP, mCherry

   Expression

   Worm

   Promoter

   -

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXF89

   Plasmid

   #139030

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   halotag (no STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEY41

   Plasmid

   #191045

   Purpose

   dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   dat-1 (dat-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY43

   Plasmid

   #191047

   Purpose

   eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   eat-4(prom6-1) (eat-4 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ1320

   Plasmid

   #84829

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Expression

   Bacterial and Worm

   Promoter

   Peft-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK2014

    Plasmid

    #171931

    Purpose

    To express sygl-1 fused to 24 copies of MS2 loops.

    Depositor

    Judith Kimble

    Article

    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

    Insert

    Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
    

    Tags

    N-terminal Tag 24X MS2 loops

    Expression

    Worm

    Promoter

    -

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXF87

    Plasmid

    #139027

    Purpose

    MosSCI backbone for MosSCI-SapTrap assembly

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRab-3::Cerulean-Venus::Lgg-1

    Plasmid

    #68045

    Purpose

    Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pRab-3::Cerulean-Venus::Lgg-1
    

    Expression

    Worm

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

    Plasmid

    #225924

    Purpose

    Expressing LGC-47 in AVA neuron

    Depositor

    Andrew Leifer

    Article

    Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

    Insert

    LGC-47 (lgc-47 Nematode)
    

    Expression

    Worm

    Promoter

    rig-3

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEM255 - sgRNA mosTI Pmlc-2_GFP

    Plasmid

    #159835

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI Pmlc-2_GFP
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLS338

    Plasmid

    #73719

    Purpose

    SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Unc-32::GFP(Cbr-unc-119)
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFS16 Pmyo-3 NPP-9 mChBioFLAG

    Plasmid

    #45099

    Depositor

    Steven Henikoff

    Article

    Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

    Inserts

    npp-9 (npp-9 Nematode)
    unc-119 (unc-119 Nematode)
    

    Tags

    BLRP, FLAG, and mCherry

    Expression

    Worm

    Promoter

    myo-3 and unc-119

    Available Since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPD_Pinx-1::ChR2(H134R)::mCherry

    Plasmid

    #117420

    Purpose

    For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Expression

    Worm

    Mutation

    H134R

    Promoter

    inx-1

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPD95_25

    Plasmid

    #1486

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, ATG-SecSig, pes10

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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