1,748 results
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Plasmid#172696PurposePrgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPrgef-1
UseTagsGFP 1-10ExpressionWormMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1586
Plasmid#121057PurposemKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmKate2-T^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized mKate2, and au…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
UseTagsExpressionWormMutationYFP with destabilized signal from mouse ornithine…PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW450
Plasmid#198831PurposeVoltage-sensitive fluorescent protein.DepositorInsert15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD382
Plasmid#91830PurposeLox2272-flanked SEC donor for the SapTrap cloning systemDepositorInsertLox2272-SEC-Lox2272
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
UseTagsGFP (S65C)ExpressionWormMutationPromotersur-5Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-GFP(dpiRNA)::CDK-1
Plasmid#107938Purposecdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertGFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
UseTagsExpressionWormMutationPromotercdk-1Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXF88
Plasmid#139039PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wemiRFP670-T2A-HO1
Plasmid#197243PurposeExpresses the protein of wemiRFP670-T2A-HO1 in neurons of C eleganDepositorInsertwemiRFP670-T2A-HO1
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionWorm and YeastMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pflp-18::NGL-1::spGFP11
Plasmid#65828PurposeFor trans-synaptic labelingDepositorInsertNLG-1 (nlg-1 Nematode)
UseTagsspGFP11ExpressionWormMutationN142SPromoterPflp-18Available sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
BSP610
Plasmid#122255Purposeexpression vector for mtagBFP2 under EFT3 promoterDepositorInsertmtagBFP2
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJH3449
Plasmid#179576PurposePacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsBFPExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNM3814
Plasmid#154122Purposemec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vectorDepositorInsertmec-4p::rtetR-L-QFAD::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA with U6 promoter
Plasmid#48962Purposeto drive the sgRNA expression under a U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pJS145
Plasmid#179159PurposeEncodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.DepositorInsertrps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
UseTagsExpressionWormMutationFlexon replacing 2nd intron of 2xNLS::GFPPromoterrps-27pAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEft-3::Cerulean-Venus::Tomm-7
Plasmid#68046PurposeUbiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.DepositorInsertpEft-3::Cerulean-Venus::Tomm-7
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only