1,737 results

1. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159857

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEft-3::Cerulean-Venus::Tomm-7

   Plasmid

   #68046

   Purpose

   Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

   Depositor

   Dana Miller

   Article

   Chapin et al Aging (Albany NY). 2015 Jun 30.

   Insert

   pEft-3::Cerulean-Venus::Tomm-7
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pflp-18::NGL-1::spGFP11

   Plasmid

   #65828

   Purpose

   For trans-synaptic labeling

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   NLG-1 (nlg-1 Nematode)
   

   Use

   Tags

   spGFP11

   Expression

   Worm

   Mutation

   N142S

   Promoter

   Pflp-18

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA with U6 promoter

   Plasmid

   #48962

   Purpose

   to drive the sgRNA expression under a U6 promoter

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ150-set2

   Plasmid

   #82546

   Purpose

   Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::set-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBN396

   Plasmid

   #109326

   Purpose

   Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

   Depositor

   Peter Askjaer

   Article

   Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

   Insert

   mex-5 (mex-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   mex-5

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR49

   Plasmid

   #69259

   Purpose

   phlh-8::tagBFP2

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   TagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, TagBFP2 contains 2 sing…

   Promoter

   hlh-8

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDD378

   Plasmid

   #91827

   Purpose

   Codon-optimized HaloTag donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   HaloTag-C1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ChrII_ttTi5605_loxP_myo2_neoR

    Plasmid

    #194056

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ttTi5605
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

    Plasmid

    #182358

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmex-5

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS235

    Plasmid

    #191855

    Purpose

    Negative selection marker for MosSCI

    Depositor

    K Subramaniam

    Article

    Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

    Insert

    peel-1 toxic protein for negative selection against extrachromosomal array
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.41

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1839

    Plasmid

    #154325

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Punc-4::NGL-1::spGFP1-10

    Plasmid

    #65827

    Purpose

    For trans-synaptic labeling

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    NLG-1 (nlg-1 Nematode)
    

    Use

    Tags

    spGFP1-10

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. XW12

    Plasmid

    #65834

    Purpose

    Pquas-Δpes10-GFP-3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    5XQUAS-delta-pes-10
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJF6

    Plasmid

    #139040

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    gfp (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TC358

    Plasmid

    #164885

    Purpose

    TRE::GFP, inducible GFP expression when co-express with rtTA

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

    Plasmid

    #200367

    Purpose

    cbr-unc-119 rescue gene for use as co-injection marker.

    Depositor

    Christian Froekjaer-Jensen

    Article

    AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

    Insert

    [cbr-unc-119(+)]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge