1,779 results

1. pEM3 = ncs-1p::nCre

   Plasmid

   #24034

   Depositor

   Cori Bargmann

   Article

   Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

   Insert

   ncs-1 (ncs-1 Nematode)
   

   Use

   Cre/Lox

   Tags

   nCre

   Expression

   Worm

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1836

   Plasmid

   #154337

   Purpose

   Promoter reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBN154

   Plasmid

   #86718

   Purpose

   FLP/Frt dual color reporter for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
   

   Expression

   Worm

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD363

   Plasmid

   #91829

   Purpose

   LoxP-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   LoxP-SEC-LoxP
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF11-wmiRFP670-2-T2A-HO1

   Plasmid

   #197250

   Purpose

   Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP670-2-T2A-HO1
   

   Expression

   Worm

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGLOW39

   Plasmid

   #173068

   Purpose

   mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   mScarlet-I-C1^SEC^3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized mScarlet

   Expression

   Worm

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSM08

   Plasmid

   #139042

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   mscarlet (includes STOP codon)
   

   Expression

   Worm

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOL2339

   Plasmid

   #37839

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Ub-V
   

   Tags

   mCherry

   Expression

   Worm

   Promoter

   nhx-2

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOL2340

    Plasmid

    #37841

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Ub-M
    

    Tags

    mCherry

    Expression

    Worm

    Mutation

    R77M

    Promoter

    nhx-2

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJW1347

    Plasmid

    #154310

    Purpose

    Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa Linker for CT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMLS134

    Plasmid

    #73714

    Purpose

    SapTrap destination vector for building sgRNA expression vectors.

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    pU6::sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    SapI insertion site

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

    Plasmid

    #107132

    Purpose

    cGAL-N driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Expression

    Worm

    Promoter

    C. elegans eft-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107136

    Purpose

    cGAL-C driver under the control of C. elegans eft-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Expression

    Worm

    Promoter

    C. elegans eft-3 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCC324

    Plasmid

    #110165

    Purpose

    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    R61K change made in ERK-KTR CDS

    Promoter

    lin-31 promoter/enhancer

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS3-rab-3P::AI::ChR2(H134R)

    Plasmid

    #232601

    Purpose

    Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    Channelrhodopsin2
    

    Expression

    Worm

    Mutation

    H134R

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ150-GFP(dpiRNA)::CDK-1

    Plasmid

    #107938

    Purpose

    cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
    

    Expression

    Worm

    Promoter

    cdk-1

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ178 - cxTi10882_MCS

    Plasmid

    #19331

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    cxTi10882 targetting region
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXF276

    Plasmid

    #139048

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    lov domain (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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