1,755 results

1. pCFJ178 - cxTi10882_MCS

   Plasmid

   #19331

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   cxTi10882 targetting region
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEft-3::Cerulean-Venus::Tomm-7

   Plasmid

   #68046

   Purpose

   Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

   Depositor

   Dana Miller

   Article

   Chapin et al Aging (Albany NY). 2015 Jun 30.

   Insert

   pEft-3::Cerulean-Venus::Tomm-7
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. XW12

   Plasmid

   #65834

   Purpose

   Pquas-Δpes10-GFP-3'UTR-unc-54

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   5XQUAS-delta-pes-10
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ1955

   Plasmid

   #84830

   Purpose

   Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized tdTomato
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

   Plasmid

   #159849

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJW1583

   Plasmid

   #121054

   Purpose

   GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   GFP^SEC^AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, C. elegans codon optimized GFP, and auxin…

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDD04neo pmyo2::gfp

   Plasmid

   #26393

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNM3724

    Plasmid

    #154112

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHZ081

    Plasmid

    #27506

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH3449

    Plasmid

    #179576

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Plgc-55B

    Available since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pKS2301

    Plasmid

    #37834

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2cpl-1::YFP (cpl-1 Nematode)
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBCN27-Pmyo-2::GFP::unc-54_3'UTR

    Plasmid

    #26347

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

    Insert

    Pmyo-2::GFP::unc-54_3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ352

    Plasmid

    #30539

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBN396

    Plasmid

    #109326

    Purpose

    Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

    Depositor

    Peter Askjaer

    Article

    Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

    Insert

    mex-5 (mex-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    mex-5

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJW1839

    Plasmid

    #154325

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDD378

    Plasmid

    #91827

    Purpose

    Codon-optimized HaloTag donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    HaloTag-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge