8,173 results

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pJW1516

Plasmid

#62386

Purpose

pFA6a Osm1(M1A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 1 to Alanine

Promoter

Adh1p

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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LPH2776 - Ub-I44A

Plasmid

#32163

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

I44A

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pKT0240

Plasmid

#8739

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Tags

13x Myc and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
sup35-R320I

Plasmid

#1199

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed arg 320 to isoleucine

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pNB788

Plasmid

#60165

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

RNR1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB691

Plasmid

#60135

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Promoter

MET17pr

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pRG227

Plasmid

#64532

Purpose

Episomal E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAG413GPD-EYFP-ccdB

Plasmid

#14334

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG303GAL-ccdB-DsRed

Plasmid

#14349

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306GAL-ccdB-Cerulean

Plasmid

#14379

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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p404GALL

Plasmid

#17418

Depositor

Nicolas Buchler, Fred Cross

Insert

GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GALL promoter is between SacI and XbaI restrictio…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-Kan

Plasmid

#44907

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Expression

Yeast

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJJH1307

Plasmid

#36910

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScLEU2

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pNB753

Plasmid

#60148

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Promoter

LEU1pr

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRPR1_c6gRNA_RPR1t

Plasmid

#64384

Purpose

encodes c6 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c6 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
p41Neo 3-F GW

Plasmid

#58554

Purpose

CEN vector with G418 resistance marker for triple Gateway insertion

Depositor

Leonid Kruglyak

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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LexA-Fus3

Plasmid

#18899

Depositor

Elaine Elion

Article

Elion et al Cell. 1990 Feb 23. 60(4):649-64.

Insert

FUS3 (FUS3 Budding Yeast)

Expression

Yeast

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
Gal4pBS24-Pleum

Plasmid

#60327

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pGLx2-set1deltaRRM

Plasmid

#53257

Purpose

GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

RRM domain deleted (aa 230-355)

Promoter

GAL1/10

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE HDEL

Plasmid

#32118

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Available Since

Oct. 12, 2011

Availability

Academic Institutions and Nonprofits only
p414GPD_Hsp82_CycTER

Plasmid

#61710

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

GPD

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAG303GPD-ccdB-ECFP

Plasmid

#14158

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGB42/Kan

Plasmid

#17261

Depositor

Stuart Schreiber

Article

Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

Type

Empty backbone

Tags

B42•HA

Expression

Yeast

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pRB306

Plasmid

#8666

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pAG306GPD-ECFP-ccdB

Plasmid

#14284

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG303GAL-ccdB-TAP

Plasmid

#14253

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

TAP

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
p416 3-F GW

Plasmid

#58552

Purpose

pRS416 carrying Gateway cassette for three inserts

Depositor

Leonid Kruglyak

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306GPD-Cerulean-ccdB

Plasmid

#14404

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB819-PKR-gib-FFA

Plasmid

#20043

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

Y489F

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pRB2070

Plasmid

#8671

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 with LYS2 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pAG423GAL-ECFP-ccdB

Plasmid

#14293

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
M3939 leu2::ADE2 Disruptor Converter

Plasmid

#51672

Purpose

Yeast marker swap plasmid for converting LEU2 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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