-
Plasmid#235753PurposeProtein expression of ScVPS30 and ZZ-ScVPS38DepositorUseTagsZZ-3xTEVExpressionYeastMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pAPInt-mNeonGreen-G418
Plasmid#236484PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO831
Plasmid#235754PurposeProtein expression of ZZ-ScVPS38 (1-212F)DepositorInsertVPS38
UseTagsZZ-3xTEVExpressionYeastMutationaa 1-212FPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO934
Plasmid#235766PurposeExpression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoterDepositorInsertVPS34 delta CBR1
UseTags3xFLAGExpressionYeastMutationaa 31-55 deletedPromoterScVPS34 promoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO225
Plasmid#235729PurposeProtin expression of FL yeast VPS15 -ZZ in yeastDepositorInsertVPS15
UseTags3xTEV-ZZExpressionYeastMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO290
Plasmid#235731PurposeProtein expression in FL yeast VPS34 D731N mutant in yeastDepositorInsertVPS34
UseTagsExpressionYeastMutationS2A, D731NPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO69
Plasmid#235747PurposeProtein expression of FL ScVPS34 in yeastDepositorInsertVPS34
UseTagsExpressionYeastMutationS2APromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO361
Plasmid#235748PurposeProtein expression of ScVPS34 HELCATDepositorInsertVPS34 HELCAT
UseTagsExpressionYeastMutationaa 268-875PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO227
Plasmid#235749PurposeProtein expression of ZZ-FL ScVPS30DepositorInsertVPS30
UseTagsZZ-3xTEVExpressionYeastMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO832
Plasmid#235755PurposeProtein expression of ScVPS30 (1-215E), untaggedDepositorInsertScVPS30
UseTagsExpressionYeastMutationaa 1-215EPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO478
Plasmid#235757PurposeExpression of FL ScVPS34-3xFLAG under its own promoterDepositorInsertVPS34
UseTags3xFLAGExpressionYeastMutationPromoterScVPS34 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO760
Plasmid#235759PurposeExpression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoterDepositorInsertVPS38 delta C2
UseTags3xFLAGExpressionYeastMutationaa 1-154 deletedPromoterScVPS38 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO761
Plasmid#235760PurposeExpression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoterDepositorInsertVSP38 delta BARA2
UseTags3xFLAGExpressionYeastMutationaa 320-440 deletedPromoterScVPS38 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO764
Plasmid#235761PurposeExpression of ScVPS38-EGFP under its own promoterDepositorInsertVPS38
UseTagsEGFPExpressionYeastMutationPromoterScVPS38 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO765
Plasmid#235762PurposeExpression of ScVPS38 delta C2 (1-154) -EGFP under its own promoterDepositorInsertVPS38
UseTagsEGFPExpressionYeastMutationaa 1-154 deletedPromoterScVPS38 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO766
Plasmid#235763PurposeExpression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoterDepositorInsertVSP38 delta BARA2
UseTagsEGFPExpressionYeastMutationaa 320-440 deletedPromoterScVPS38 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO932
Plasmid#235764PurposeExpression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoterDepositorInsertVPS34 HELCAT
UseTags3xFLAGExpressionYeastMutationaa 268-875PromoterScVPS34 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO933
Plasmid#235765PurposeExpression of ScVPS34 delta C154-V202-3xFLAG under its own promoterDepositorInsertVPS34 delta C154-V202
UseTags3xFLAGExpressionYeastMutationaa 154-202 deletedPromoterScVPS34 promoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO503
Plasmid#235743PurposeProtein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genomeDepositorInsertKmTOR
UseTagsZZ-3xTEVExpressionYeastMutationaa 4-4609PromoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO524
Plasmid#235744PurposeProtein expression of KmLst8 by integrating KmLST8 into the K. marxianus genomeDepositorInsertKmLST8
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRW404-2µ-BXB1
Plasmid#235976Purpose2µ plasmid for constitutive expression of BXB1 under RNR2 promoter.DepositorInsertBXB1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYY10-2µ-TP901
Plasmid#235771Purpose2µ plasmid for constitutive expression of TP901 under RNR2 promoter.DepositorInsertTP901 integrase
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-RWT4Ca_Reg1
Plasmid#237525PurposeYeast two-hybridDepositorInsertRwt4Ca_Reg1
UseTagsExpressionYeastMutationRemoved binding region 1PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-RWT4Ca+C-Tail
Plasmid#237526PurposeYeast two-hybridDepositorInsertRwt4Ca+C-Tail
UseTagsExpressionYeastMutationAdd C-terminal extensionPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-RWT4Ca
Plasmid#237524PurposeYeast two-hybridDepositorInsertRwt4Ca
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-AvrPWT4
Plasmid#237527PurposeYeast two-hybridDepositorInsertAvrPwt4
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-VirPWT4
Plasmid#237528PurposeYeast two-hybridDepositorInsertVirPwt4
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-NET1
Plasmid#232893PurposePlasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.DepositorInsertNET1 gRNA (NET1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-KAE1
Plasmid#232891PurposePlasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.DepositorInsertKAE1 gRNA (KAE1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-VRG4
Plasmid#232899PurposePlasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.DepositorInsertVRG4 gRNA (VRG4 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SAM50
Plasmid#232895PurposePlasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.DepositorInsertSAM50 gRNA (SAM50 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold2s
Plasmid#231761PurposeExpression of mGold2s in yeast cellsDepositorInsertmGold2s
UseTagsExpressionYeastMutationmGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterpTDH(GAP promoter)Available sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-SoxN-A22-EGFP
Plasmid#181731PurposeGalactose inducible expression of SoxN-A22-EGFPDepositorInsertSoxN-A22-EGFP (SoxN Fly)
UseTags3xFLAG-EGFPExpressionYeastMutationextended repeat of 22 Ala residues replacing nati…PromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
P415 ymNeongreen
Plasmid#224110PurposeExpressing ymNeongreen in yeastDepositorInsertymNeongreen
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::TRP1
Plasmid#227593PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::TRP1
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::LEU2
Plasmid#227594PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::LEU2
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
P415 ymBaojin
Plasmid#224086PurposeExpressing ymBaojin in yeastDepositorInsertymBaoJin
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMY.PRC2
Plasmid#234582PurposeExpression of ProtA-EZH2, SUZ12, EED and RBBP4DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Hyg
Plasmid#236486PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.DepositorInsertmStayGold
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Nat
Plasmid#236485PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.DepositorInsertmStayGold
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only