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8,173 results

Showing: 5721 - 5760 of 8173 results

  1. pGBT9-Hook1

    Plasmid
    #198523
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979
    Insert
    Hook1 (HOOK1 Human)
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    silent substitution in codon 620 to eliminate int…
    Promoter
    ADH1
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198523-plasmid-map-sequence-id-389375

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  2. pGADT7-δ

    Plasmid
    #197259
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-3 δ (AP3D1 Human)
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Val 549 Ile substitution; silent substitutions in…
    Promoter
    ADH1
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197259-plasmid-map-sequence-id-387016

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  4. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

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  6. pGADT7-AP-4 β4

    Plasmid
    #198327
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-4 β4
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Contains L480S substitution
    Promoter
    ADH1
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198327-plasmid-map-sequence-id-387831

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  8. pGADT7-AP-2 σ2

    Plasmid
    #198345
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-2 σ2
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    R42G substitution, and silent substitutions in co…
    Promoter
    ADH1
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198345-plasmid-map-sequence-id-387832

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  9. pGADT7-AP-1 σ1B

    Plasmid
    #198335
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1B
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198335-plasmid-map-sequence-id-387734

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  10. pGADT7-AP-1 σ1A

    Plasmid
    #198334
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1A
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198334-plasmid-map-sequence-id-387458

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  11. pGADT7-AP-1 σ1C

    Plasmid
    #198336
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1C
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    S148C substitution
    Promoter
    ADH1
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198336-plasmid-map-sequence-id-387637

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  16. pACT2-μ1A

    Plasmid
    #198168
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1A
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198168-plasmid-map-sequence-id-387525

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  17. pACT2-μ2

    Plasmid
    #198173
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-2 μ2
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198173-plasmid-map-sequence-id-387524

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  22. pGADT7-γ1

    Plasmid
    #197078
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-1 γ1 (Ap1g1 Mouse)
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197078-plasmid-map-sequence-id-387046

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  23. pGADT7-ε

    Plasmid
    #197261
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-4 ε (AP4E1 Human)
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitutions in codons 905, 1129 and 1137
    Promoter
    ADH1
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197261-plasmid-map-sequence-id-387043

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  30. pCAS-mak10 gRNA-MAK10Δ

    Plasmid
    #160384
    Purpose
    For CRISPR-mediated deletin og MAK10 in yeast
    Depositor
    John McCusker
    Insert
    MAK10
    Expression
    Yeast
    Promoter
    PGK1
    Available Since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    160384-plasmid-map-sequence-id-384173

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  31. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Expression
    Yeast
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

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  38. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  40. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial and Yeast
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

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Showing: 5721 - 5760 of 8173 results