8,432 results

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pYES2.1-pomMet14

Plasmid

#107451

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Publication

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met14 (met14 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-Erg11-iGFPx3

Plasmid

#115422

Purpose

Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Erg11-iGFPx3

Tags

iGFP

Expression

Yeast

Promoter

Endogenous

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIplac204-pOst1-ESCargo

Plasmid

#115420

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Publication

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Expression

Bacterial and Yeast

Promoter

ATG41 endogenous promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR217

Plasmid

#113872

Purpose

expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Publication

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA9-MPH2-3 sgRNA10-MAL11

Use

CRISPR

Expression

Yeast

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDTN3997

Plasmid

#113337

Purpose

Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

Depositor

Suzanne Sandmeyer

Publication

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

multi-copy 2-pyrone synthase

Expression

Yeast

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSir1

Plasmid

#107447

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Publication

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sir1 (sir1 Fission Yeast)

Tags

V5-His

Expression

Yeast

Mutation

H523R, A750T

Promoter

GAL1

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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yCp50-PRP2

Plasmid

#111304

Purpose

Prp2 in yCp50 plasmid

Depositor

Christine Guthrie

Publication

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP2

Expression

Yeast

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSE360-Prp22

Plasmid

#111284

Purpose

Prp22 WT gene in pSE360 backbone

Depositor

Christine Guthrie

Publication

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP22

Expression

Yeast

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHM4 (U6 in pSE360)

Plasmid

#111281

Purpose

U6 snRNA coding sequence in pSE360(Ura) plasmid

Depositor

Christine Guthrie

Publication

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

SNR6

Expression

Yeast

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG418

Plasmid

#105342

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK011

Plasmid

#105343

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK012

Plasmid

#105344

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD14

Plasmid

#112822

Purpose

pSMF2 with 14 CTD repeats

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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Sub2 pRS315

Plasmid

#111300

Purpose

Sub2 in pRS315 vector

Depositor

Christine Guthrie

Publication

Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

Insert

SUB2

Expression

Yeast

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316-SNU114

Plasmid

#111308

Purpose

pRS316-SNU114

Depositor

Christine Guthrie

Publication

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Expression

Yeast

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCSJ125

Plasmid

#104344

Purpose

Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Publication

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

MDH2

Tags

3xFLAG

Expression

Yeast

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS313-Sad1-Wt

Plasmid

#90308

Purpose

yeast expression

Depositor

Christine Guthrie

Publication

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

no tag

Expression

Yeast

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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