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pAG413GPD-ccdB-DsRed

Plasmid

#14358

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046207

Plasmid

#145559

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pITI1

Plasmid

#166094

Purpose

Gal inducible expression of dCas9-FokI

Depositor

Rebecca Burgess

Insert

dCas9-FokI

Use

CRISPR

Expression

Yeast

Mutation

spCas9-D10A, H840A

Promoter

Gal-inducible

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAG426GPD-ccdB-ECFP

Plasmid

#14180

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pAV0543

Plasmid

#133493

Purpose

Expression of nucleo-cytoplasmic mCherry in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pVG48

Plasmid

#164706

Purpose

rtTA under the control of PGK1 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

PGK1pr - rtTA - CYC1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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JME4393

Plasmid

#129657

Purpose

LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-PmCDA1

Plasmid

#79612

Purpose

Expresses PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

PmCDA1

Expression

Yeast

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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Desmin/pAS2-1

Plasmid

#97407

Purpose

Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

Desmin (DES Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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p406MET25

Plasmid

#17421

Depositor

Nicolas Buchler, Fred Cross

Insert

MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

MET25 promoter is between SacI and XbaI restricti…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Expression

Yeast

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAG414GAL-ccdB-DsRed

Plasmid

#14359

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB1-Cterminal6xHis

Plasmid

#60387

Purpose

Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Tags

6xHIS

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pAG306GAL-ccdB-EYFP

Plasmid

#14211

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046418

Plasmid

#149029

Purpose

Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FC857

Plasmid

#27327

Depositor

Fred Cross

Article

Cross et al Yeast. 2011 Feb . 28(2):167-79.

Insert

CDH1 (CDH1 Budding Yeast)

Expression

Yeast

Mutation

C-terminal truncation of CDH1 at its BamHI site

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pKT0174

Plasmid

#8720

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-HPH

Plasmid

#160207

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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