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pFTK014

Plasmid

#171286

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PAnHisB AN6536.2 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK012

Plasmid

#171284

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P40s An0465 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK010

Plasmid

#171282

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PxlnA ANIA_03613 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK008

Plasmid

#171280

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Poat1 Pc18g03600 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK007

Plasmid

#171279

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PfraA An16g04690 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK006

Plasmid

#171278

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Ptef EF1-subunit ANIA_02063 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK005

Plasmid

#171277

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Ptef1 ANIA_04218 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK003

Plasmid

#171275

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Patp9 An04g08190 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTK002

Plasmid

#171274

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgndA An11g02040 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK001

Plasmid

#171273

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Pact Pc20g11630 promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Use

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER

Plasmid

#52686

Purpose

Same as 41024, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-3xHA+LINKER

Use

Promoter replacement

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-eGFP

Plasmid

#126720

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-LacI-Mit1AD

Plasmid

#126722

Purpose

LacI-Mit1AD on pGAPZ_ A expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2

Plasmid

#60388

Purpose

Expression of yeast beta tubulin (TUB2) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Use

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAV0586

Plasmid

#133473

Purpose

Integration into fission yeast genome at lys3 locus with bsdMX selection cassette

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-mVenus-FLAG-LANS1-Set2

Plasmid

#122003

Purpose

mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

mVenus-FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAH233

Plasmid

#121144

Purpose

Expression of gRNA with BbsI placeholder for target cloning

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

gRNA cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

S.pombe rrk1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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