Skip to main content
Addgene
Showing: 301 - 320 of 7821 results

  5. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

    Enlarge

  9. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Insert
    Cas3
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229538-plasmid-map-sequence-id-459007

    Enlarge

  10. FRP2370

    Plasmid
    #127576
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127576-plasmid-map-sequence-id-252228

    Enlarge

  11. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

    Enlarge

  12. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

    Plasmid
    #99049
    Purpose
    Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain
    Depositor
    Barak Cohen
    Article
    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1
    Inserts
    ACT1 promoter (ACT1 Budding Yeast)
    mCherry
    Zif268 DNA binding Domain
    Estrogen response domain (ESR1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    99049-plasmid-map-sequence-id-350965

    Enlarge

  13. pGAL-PDE1

    Plasmid
    #160388
    Purpose
    Overxpression of PDE1 in yeast
    Depositor
    John McCusker
    Insert
    PDE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160388-plasmid-map-sequence-id-314523

    Enlarge

  19. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GalL
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

    Enlarge

  20. FRP2365

    Plasmid
    #127575
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127575-plasmid-map-sequence-id-252224

    Enlarge

Showing: 301 - 320 of 7821 results