8,085 results

1. pAW240

   Plasmid

   #170791

   Purpose

   P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

   Depositor

   Chang Liu

   Article

   Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

   Insert

   p10B2-AT110
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   10B2 (P1 specific promoter)

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p404ADH1

   Plasmid

   #15970

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   ADH1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG425GPD-ccdB

   Plasmid

   #14154

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-6xGLY-V5-hphMX4

   Plasmid

   #20779

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoEGFP-Kan

   Plasmid

   #44900

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGS_101

   Plasmid

   #160541

   Purpose

   Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

   Depositor

   Li-Huei Tsai, Susan Lindquist

   Article

   Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

   Insert

   Apolipoprotein E e4 (APOE Human)
   

   Use

   Tags

   Kar2 signal sequence

   Expression

   Yeast

   Mutation

   e4 allele

   Promoter

   GAL1

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pG1

   Plasmid

   #162602

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lact-C2-GFP-p416

   Plasmid

   #22853

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pMetYC-Dest

   Plasmid

   #105081

   Purpose

   Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Use

   Tags

   Cub-PLV

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG413GPD-ccdB

    Plasmid

    #14142

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-6xGLY-V5-kanMX6

    Plasmid

    #20780

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG414GPD-ccdB

    Plasmid

    #14144

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pANCH2-Nat

    Plasmid

    #87249

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. miniTurbo-V5_pRS415

    Plasmid

    #107168

    Purpose

    expresses V5-tagged miniTurbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEmpty_Control

    Plasmid

    #162607

    Purpose

    Empty control for Cas9 experiments and to clone new guide RNAs for paper

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

    Plasmid

    #58437

    Purpose

    Expresses LexA-ER-B112 in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    LexA-ER-B112
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Act1 promoter (-520;1)

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-mCherry-hphMX6

    Plasmid

    #105156

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    mCherry-hphMX6
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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