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pGBW-m4046442
Plasmid#145550PurposeYeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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LHP585 - Ub-CYC1
Plasmid#32168DepositorUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pDSM-de-Pfba1-PYC-Trpl15a
Plasmid#127738PurposeYeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceJan. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK273
Plasmid#71563PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeastDepositorInsertdelta12 fatty acid desaturase (form 2)
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal tr-lux
Plasmid#1330DepositorInsertBacterial luciferase-V.h.CTP5
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mYFP
Plasmid#105141PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mYFP
UseTags41nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter41nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXRH3
Plasmid#63145PurposeHis3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYEXC3H
Plasmid#49026PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionYeastMutationPromoterGAL1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-EYFP-ccdB
Plasmid#14341DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNC891
Plasmid#20397DepositorInsertYFP-reporter
UseTagsExpressionYeastMutationX=M (see schematic)PromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
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YIplac211-KEX2-iGFPx6
Plasmid#105264PurposePlasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.DepositorInsertKex2 portion (KEX2 )
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVG46
Plasmid#164704PurposeyEVenus under the control of PHO5 promoterDepositorInsertPHO5pr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F13
Plasmid#23056DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only
