8,133 results

1. pILGFP9E3

   Plasmid

   #185842

   Purpose

   Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS406 ATG1-ATG17-3'UTR

   Plasmid

   #166848

   Purpose

   Expresses Atg1 with ATG17 3'UTR in yeasts.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   ATG1 Promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046619

   Plasmid

   #149218

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. LD102

   Plasmid

   #69161

   Purpose

   Integration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg2-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ377

   Plasmid

   #74214

   Purpose

   Combined sgRNA/Cas9 vector with LEU2 marker

   Depositor

   Mikel Zaratiegui

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046219

   Plasmid

   #153926

   Purpose

   Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

   Plasmid

   #184407

   Purpose

   yeast surface display of the SARS-CoV-2 Delta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   L452R+T478K

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPTK016-3-RFP_Intra

   Plasmid

   #84975

   Purpose

   Type 3, Red fluorescent protein

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   BsaI site removed (1677 c to g and 1794 a to g)

   Promoter

   Non

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS426-GFP-Num1-PH

   Plasmid

   #71374

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pEDJ437

    Plasmid

    #177270

    Purpose

    USER cloning-compatible 2μ vector (HIS3)

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAB42

    Plasmid

    #17268

    Depositor

    Stuart Schreiber

    Article

    Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

    Type

    Empty backbone

    Tags

    B42•HA

    Expression

    Yeast

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pJT131_GalL_cCDA1-NGBE3

    Plasmid

    #145095

    Purpose

    Expressing base editor cCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-NGBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046217

    Plasmid

    #149071

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. U6-U57C

    Plasmid

    #169928

    Purpose

    WT U6 with a U57C mutation

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    U6 U57C
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK218TK620T

    Plasmid

    #1192

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed lysine 218 to threonine and lysine 620 to…

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM2_12

    Plasmid

    #183061

    Purpose

    Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMM2_4

    Plasmid

    #183057

    Purpose

    HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mRuby2
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mRuby2 and mTurquoise2

    Expression

    Yeast

    Promoter

    HAA1 and YGP1

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM2_10

    Plasmid

    #183059

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM2_9

    Plasmid

    #183058

    Purpose

    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM2_11

    Plasmid

    #183060

    Purpose

    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

    Inserts

    mCherry
    HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Promoter

    HAA1 and Synthetic promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge