8,351 results
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Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPeft2AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPsmtef1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pfba1p-ACO-Tgpm1
Plasmid#127731PurposeYeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPfba1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pykt6-ACO-Tprm9
Plasmid#127732PurposeYeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPykt6AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Ptef1-ACO-Tyol036w
Plasmid#127733PurposeYeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPtef1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPsptdh3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyExpressionYeastPromoterPpgk1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyExpressionYeastPromoterPpxr1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ptef1-CSC-Tpdc1
Plasmid#127739PurposeYeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyExpressionYeastPromoterPfba1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Pzuo1-CSC-Tvma16
Plasmid#127740PurposeYeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyExpressionYeastPromoterPzuo1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Ppfy1-CSC-Taip1
Plasmid#127741PurposeYeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyExpressionYeastPromoterPpfy1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyExpressionYeastPromoterPsbtdh3AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Peno2-ACDH-Ttal1
Plasmid#127743PurposeYeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPeno2AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Pvma6-ACDH-Trps14a
Plasmid#127744PurposeYeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPvma6AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Prpl28-ACDH-Tecm10
Plasmid#127745PurposeYeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPrpl28AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Ptdh3-ACDH-Trpl3
Plasmid#127746PurposeYeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyExpressionYeastPromoterPtdh3AvailabilityAcademic Institutions and Nonprofits only -
pEMY01CD-TER
Plasmid#127751PurposeIntegrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pAW
Plasmid#133893PurposeExpression of Gal4AD-Prey hybrid proteinDepositorTypeEmpty backboneExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pBW
Plasmid#133897PurposeExpression of Gal4BD-Bait hybrid protein.DepositorTypeEmpty backboneExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-Lam
Plasmid#133902PurposeNegative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorAvailabilityAcademic Institutions and Nonprofits only -
pMS22H-IRE
Plasmid#133905PurposePositive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWHDepositorAvailabilityAcademic Institutions and Nonprofits only -
pJW1669
Plasmid#112043PurposeCEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URADepositorInsertNSG2
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1672
Plasmid#112046PurposeCEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URADepositorInsertPST1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1674
Plasmid#112048PurposeCEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URADepositorInsertSWP1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-MET17
Plasmid#122885PurposeADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-LEU2
Plasmid#122888PurposeLYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-HIS3
Plasmid#122891PurposeTRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-MET17
Plasmid#122893PurposeTRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-MET17
Plasmid#122910PurposeLYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-URA3
Plasmid#122911PurposeLYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-LEU2
Plasmid#122913PurposeTRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-MET17
Plasmid#122914PurposeTRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-KanMx
Plasmid#122920PurposeLYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
pHES942
Plasmid#90408PurposeExpresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-SIC1
ExpressionYeastAvailabilityAcademic Institutions and Nonprofits only -
Sub2-3 in pRS314
Plasmid#90454PurposeyeastDepositorInsertSUB2
ExpressionYeastMutationsub2-3 mutationAvailabilityAcademic Institutions and Nonprofits only -
Sub2-4 in pRS314
Plasmid#90455PurposeyeastDepositorInsertSUB2
ExpressionYeastMutationsub2-4 mutationAvailabilityAcademic Institutions and Nonprofits only -
Sub2-6 in pRS314
Plasmid#90456PurposeyeastDepositorInsertSUB2
ExpressionYeastMutationsub2-6 mutationAvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
ExpressionYeastPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pHCMM2
Plasmid#167453PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoterDepositorInsertPSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
ExpressionYeastPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
pHCMM3
Plasmid#167454PurposeA control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]DepositorInsertPSEC63_yeGFP_cePUP-2_URA3_TADH1
ExpressionYeastPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
WT Ecm2
Plasmid#169922PurposeEcm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416DepositorInsertEcm2
ExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-143
Plasmid#169923PurposeWT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2DepositorInsertEcm2 1-143
ExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-265
Plasmid#169925PurposeWT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2DepositorInsertEcm2 1-265
ExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-325
Plasmid#169926PurposeWT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2DepositorInsertEcm2 1-325
ExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 L306P
Plasmid#169262PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306PDepositorInsertNop4 L306P (NOP4 Budding Yeast)
Tags3xFLAGExpressionYeastMutationp.(Leu306Pro)PromoterGPDAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 dE5
Plasmid#169263PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5DepositorInsertNop4 delta E5 (NOP4 Budding Yeast)
Tags3xFLAGExpressionYeastMutationdeleted region corresponding to human Exon 5PromoterGPDAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 dE8
Plasmid#169264PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8DepositorInsertNop4 delta E8 (NOP4 Budding Yeast)
Tags3xFLAGExpressionYeastMutationdeleted region corresponding to human Exon 8PromoterGPDAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-RBM28 WT
Plasmid#169265PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WTDepositorAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-RBM28 L351P
Plasmid#169266PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351PDepositorAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-RBM28 dE5
Plasmid#169267PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5DepositorInsertRBM28 delta E5 (RBM28 Human)
Tags3xFLAGExpressionYeastMutationdeleted Exon 5PromoterGPDAvailabilityAcademic Institutions and Nonprofits only