8,128 results

1. pGBW-m4046300

   Plasmid

   #149043

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046182

   Plasmid

   #149019

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pBW105

   Plasmid

   #111137

   Purpose

   For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

   Depositor

   Alexander Varshavsky

   Article

   Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

   Insert

   MLS-ratAANAT-3FLAG (Aanat Rat)
   

   Tags

   3x Flag

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS2-1-Esf1

   Plasmid

   #99987

   Purpose

   Express yeast Esf1 with Y2H binding domain fusion

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Esf1 (ESF1 Budding Yeast)
   

   Tags

   Y2H binding domain

   Expression

   Yeast

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG303GPD-ccdB-TAP

   Plasmid

   #14254

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRF1-GW ymTurquoise2-T2A-mCherry

   Plasmid

   #118442

   Purpose

   Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2-T2A-mCherry
   

   Expression

   Yeast

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp ADE2-KanMx

   Plasmid

   #122917

   Purpose

   ADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Expression

   Yeast

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCTD26-S>A10-17

   Plasmid

   #112826

   Purpose

   pSMF2 with all S to A mutations in only repeats 10-17.

   Depositor

   Stephen Fuchs

   Article

   Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   pSMF2 with all S to A mutations in only repeats 1…

   Promoter

   Native promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTD26-S>A2-9

   Plasmid

   #112825

   Purpose

   pSMF2 with all S to A mutations in only repeats 2-9.

   Depositor

   Stephen Fuchs

   Article

   Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   pSMF2 with all S to A mutations in only repeats 2…

   Promoter

   Native promoter

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCTD26-S>A18-25

    Plasmid

    #112827

    Purpose

    pSMF2 with all S to A mutations in only repeats 18-25.

    Depositor

    Stephen Fuchs

    Article

    Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    pSMF2 with all S to A mutations in only repeats 1…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p2HGPD/ER/cyc

    Plasmid

    #1283

    Depositor

    Susan Lindquist

    Article

    Kimura et al Science 1995 Jun 2;268(5215):1362-5.

    Insert

    ER
    

    Tags

    Gpd promoter

    Expression

    Yeast

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pRS423-SgH

    Plasmid

    #163972

    Purpose

    Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXP812

    Plasmid

    #46062

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJJH1242

    Plasmid

    #36909

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScURA3
    

    Use

    Lentiviral

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRP322

    Plasmid

    #159303

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized CitrineA206K under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    V2del, S3del, F64L, A206K, L231H

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDRF1-GW mRuby2-T2A-mTurquoise2

    Plasmid

    #118429

    Purpose

    Produces equimolar expression of mRuby2 and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mRuby2-T2A-mTurquoise2
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXP731

    Plasmid

    #46057

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG425GAL-ECFP-ccdB

    Plasmid

    #14297

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LD109

    Plasmid

    #69164

    Purpose

    Integration of GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg9-EGFP
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GAL HA25QDProCFPp303

    Plasmid

    #15578

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only