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LPH2782 - Ub-K48R

Plasmid

#32161

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

K48R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pEM09

Plasmid

#135479

Purpose

Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Tags

GFP and hph

Expression

Bacterial and Yeast

Promoter

Spun Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pYC34

Plasmid

#63915

Purpose

Recipient overexpressing vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDS1885

Plasmid

#177341

Purpose

Expresses Aequorin in Candida albicans using the dominant marker SAT1

Depositor

Dominique Sanglard

Article

Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

Insert

Aequorin

Expression

Yeast

Promoter

ACT1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pINER5

Plasmid

#185895

Purpose

In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

Depositor

Claudia Vickers

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2

Expression

Yeast

Mutation

None

Available Since

Aug. 25, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046483

Plasmid

#153868

Purpose

Yeast expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB4666

Plasmid

#106144

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

CYC1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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