8,057 results

1. pFA6a-link-ymTq2-URA3

   Plasmid

   #168057

   Purpose

   Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   ymTurquoise2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

   Plasmid

   #58435

   Purpose

   Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)8-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-link-yoTagRFP-T-Kan

   Plasmid

   #44906

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoTagRFP-T
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMY.PRC2

   Plasmid

   #234582

   Purpose

   Expression of ProtA-EZH2, SUZ12, EED and RBBP4

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAR-Ec611

   Plasmid

   #130872

   Purpose

   A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

   Depositor

   Chang Liu

   Article

   Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

   Insert

   TP-DNAP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   I777K, L900S

   Promoter

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFM689

   Plasmid

   #74635

   Purpose

   alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Inserts

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. yHS1-M7A

   Plasmid

   #159151

   Purpose

   Cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   yHS1-M7A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of yHS1 to A…

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pYX142-mtGFP

   Plasmid

   #45050

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRPR1_gRNA_handle_RPR1t

   Plasmid

   #49014

   Purpose

   gRNA expression empty vector (yeast)

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pRPR1

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ube2w

    Plasmid

    #31432

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2W Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid

    #203510

    Purpose

    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    poly SLEV NS2B-GS-NS3 (POLY )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBT9-ubiquitin

    Plasmid

    #196999

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    ubiquitin (UBC Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PRS415-Gal1pr-sctetR-FokI

    Plasmid

    #44756

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    sctetR-FokI (fokI Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1pr

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

    Plasmid

    #116884

    Purpose

    FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

    Depositor

    Brian Kuhlman

    Article

    Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

    Insert

    LINXa4(RLR)-Bre1
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

    Promoter

    ADH1

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_D17_hrGFP

    Plasmid

    #84616

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pV1093:SpRY

    Plasmid

    #172188

    Purpose

    Genome editing of C. albicans

    Depositor

    Doug Bernstein

    Article

    Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. yHS1-M7A,H102A

    Plasmid

    #159152

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1-M7A,H102A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pTEF:ATP

    Plasmid

    #92179

    Purpose

    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    

    Use

    Integration and expression into yeast.

    Tags

    Yeast codon optimized mseCFP (donor) and cp173-mV…

    Expression

    Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-link-yomKate2-SpHis5

    Plasmid

    #44852

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge