8,057 results

1. pB_tetO7_sfGFP

   Plasmid

   #196616

   Purpose

   Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   Superfolder GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   tetO7 promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB4906

   Plasmid

   #106150

   Purpose

   EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGR596

   Plasmid

   #213787

   Purpose

   Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   See manuscript

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SJZ5-HPH

   Plasmid

   #228258

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   hph
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SSA1 S.c.

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM151

   Plasmid

   #133643

   Purpose

   Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-COX4-GFP (COX4 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. JME4580

   Plasmid

   #129659

   Purpose

   HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYZ462

   Plasmid

   #187970

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGI3EM17

   Plasmid

   #210569

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pG14-MS2-GFP

   Plasmid

   #27117

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2-binding protein
   

   Use

   Tags

   GFP, HA, and NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yomRuby2-SpHis5

    Plasmid

    #44858

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-yomCherry-Kan

    Plasmid

    #44903

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

    Plasmid

    #86418

    Purpose

    ONM/ER split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP1-10-Scs2TM
    

    Use

    Tags

    GFP1-10

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SZ_EC_02

    Plasmid

    #155341

    Purpose

    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

    Depositor

    Jens Nielsen

    Article

    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

    Inserts

    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    

    Use

    Easycloneyali insert vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    gapdh, pyk1, and tef1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCETF

    Plasmid

    #19783

    Depositor

    Jumi Shin

    Article

    Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

    Type

    Empty backbone

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG306GPD-ccdB

    Plasmid

    #14140

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCETT

    Plasmid

    #19782

    Depositor

    Jumi Shin

    Article

    Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

    Type

    Empty backbone

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM27

    Plasmid

    #133641

    Purpose

    Integration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Pho8 (PHO8 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJRoC30_VP8H

    Plasmid

    #188062

    Purpose

    PROSS-designed versatile peroxidase 8H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    38 PROSS mutations, described in publication

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB819-PKR-hum

    Plasmid

    #20030

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    Protein kinase R (EIF2AK2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge