8,086 results
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Plasmid#106150PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistanceDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHLUM (version 2)
Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF3038
Plasmid#26850DepositorInsertCreA recombinase
UseCre/LoxTagsExpressionYeastMutationN/APromoterAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP8H
Plasmid#188062PurposePROSS-designed versatile peroxidase 8HDepositorInsertPROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationPromoterAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
SZ_EC_02
Plasmid#155341PurposeOverexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XRDepositorInsertstef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
UseEasycloneyali insert vectorTagsExpressionYeastMutationPromotergapdh, pyk1, and tef1Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PR50
Plasmid#84905Purposeinduce expression of dipeptide repeat PR in yeastDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB
Plasmid#14155DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRR-AR
Plasmid#73045PurposeConstitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)DepositorInsertAndrogen receptor (AR Human)
UseTagsExpressionYeastMutationplease see depositor comments belowPromoterglyceraldehyde phosphate dehydrogenase (GPD)Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-His3MX6
Plasmid#41598DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionYeastMutationPromoterTEFAvailable SinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUDP002
Plasmid#103872PurposeBroad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)DepositorInsertshph
Spcas9 D147Y P411T
UseCRISPRTagsNLSExpressionYeastMutationD147Y P411TPromoterArxula adeninivorans TEF1 and Ashbya gossypii (Er…Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
ymScarlet
Plasmid#111917Purposeyeast optimized mScarletDepositorInsertymScarlet
UseTagsExpressionYeastMutationPromoterGPDAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426 Gal-RNQ1-YFP
Plasmid#18686DepositorAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pAG415GAL-ccdB
Plasmid#14145DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only