8,173 results

1. SIVu

   Plasmid

   #81089

   Purpose

   Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p405ADH1

   Plasmid

   #15966

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p405ADH1 (ADH1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   ADH1 promoter is between SacI and XbaI restrictio…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKmK.C2

   Plasmid

   #125060

   Purpose

   Storage plasmid for K.marxianus centromere

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   KmARS/CEN5
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKmK.T5

   Plasmid

   #125057

   Purpose

   Storage plasmid for K.marxianus terminator; type 4 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   PGK1 terminator
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKmK.P16

   Plasmid

   #125049

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   FBA1 promoter
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIU211

   Plasmid

   #44036

   Depositor

   Dane Wittrup

   Article

   Boder et al Nat Biotechnol. 1997 Jun;15(6):553-7.

   Insert

   AGA1
   

   Expression

   Yeast

   Available Since

   April 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKmK.I3R

   Plasmid

   #125065

   Purpose

   Storage plasmid for K.marxianus right homology arm

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   Right homology arm for targeting integration to SWF1/ARO1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKmK.I1R

   Plasmid

   #125063

   Purpose

   Storage plasmid for K.marxianus right homology arm

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   Right homology arm for targeting integration to LAC4
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKmK.I1L

   Plasmid

   #125030

   Purpose

   Storage plasmid for K.marxianus left homology arm; type 1 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   Left homology arm for targeting integration to LAC4
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKmK.I3L

    Plasmid

    #125032

    Purpose

    Storage plasmid for K.marxianus left homology arm; type 1 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Left homology arm for targeting integration to SWF1/ARO1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZ-pre-Ost1-alphaf(I)-Btl2

    Plasmid

    #117667

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-Btl2
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pANCH2-Nat

    Plasmid

    #87249

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Expression

    Yeast

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCASB

    Plasmid

    #190175

    Purpose

    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

    Depositor

    Jessica Tyler

    Article

    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

    Insert

    sgRNA cloning site
    

    Expression

    Bacterial and Yeast

    Mutation

    Added 5'-agagacc-3' upstream and 5'…

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKmK.C3

    Plasmid

    #125061

    Purpose

    Storage plasmid for K.marxianus centromere

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    KmARS/CEN6
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGal-BE3

    Plasmid

    #172409

    Purpose

    Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GALL

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PRS303-240xtetO

    Plasmid

    #44754

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    240x tetO array and CHRI homology
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSN075

    Plasmid

    #166732

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-link-ymScarletI3-Kan

    Plasmid

    #224077

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Tags

    ymScarletI3

    Expression

    Yeast

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid

    #181707

    Purpose

    Galactose inducible expression of Kar2-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Kar2-Beta-Amyloid
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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