8,418 results
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Plasmid#177924PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoterDepositorPublicationInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
ExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterTDH3Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
ExpressionYeastPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPICZc-HsACTB*-thymosinB-8His
Plasmid#111146PurposeExpresses human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integrationTagsThymosin beta and a His-tagExpressionYeastPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUDP025
Plasmid#103874PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactisDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
UseCRISPRExpressionYeastPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3405
Plasmid#106166PurposeEasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mEGFP-AID
Tags3xMyc and truncated AID (71-114)ExpressionYeastPromoterADH1 and TEF1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PA50
Plasmid#84906Purposeinduce expression of dipeptide repeat PA in yeastDepositorAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG416-Gal-GA50
Plasmid#84903Purposeinduce expression of dipeptide repeat GA in yeastDepositorAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM701
Plasmid#74642Purposealias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRG206MX
Plasmid#64536PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIYC08
Plasmid#64743PurposeYeast expression plasmid: acsL641P, ALD6, ADH2, ERG10DepositorInsertADH2, ALD6, acsL641P, ERG10
ExpressionYeastAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC519
Plasmid#62278PurposeYeast dCas9-VP64 expression plasmidDepositorInsertdCas9-VP64
TagsHas a VP64 fusionExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA-his-HYB
Plasmid#64333Purposehis3 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of his3 deletion gRNA cassette
ExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-leu-HYB
Plasmid#64332Purposeleu2 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of leu2 deletion gRNA cassette
ExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJZC518
Plasmid#62277PurposeYeast dCas9 expression plasmidDepositorInsertdCas9
ExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTy1mhis3-AI or pGTy1his3-AI
Plasmid#62228PurposeA Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.DepositorInsertTy1H3 retrotransposon
ExpressionYeastPromoterGAL1Available sinceFeb. 13, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pMZ288
Plasmid#59897PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI siteAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
HC078 (3xHA-Atg13)
Plasmid#59544PurposeExpresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
Tags3xHAExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1
Plasmid#58432PurposeInduces Citrine expression from a promoter containing 1 lexA box in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by