8,057 results

1. pILGFP3AA5

   Plasmid

   #185875

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ158

   Plasmid

   #98414

   Purpose

   Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM112B

   Plasmid

   #210602

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
   

   Use

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2A/Bpro (divergent)

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p404TEF1

   Plasmid

   #15972

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   TEF1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG4

   Plasmid

   #162605

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSS146

   Plasmid

   #64770

   Purpose

   For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   Cre-EBD78
   

   Use

   Tags

   fused to the human Estrogen Binding Domain (EBD),…

   Expression

   Yeast

   Mutation

   Promoter

   TDH4

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MS2-Snapf

   Plasmid

   #65860

   Purpose

   Expresses RNA-binding protein MS2 with fast SNAP fusion tag

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

   Inserts

   MS2
   Fast SNAP
   

   Use

   Tags

   HA and NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p414GPD_Hsp82viiB

   Plasmid

   #61709

   Purpose

   Yeast plasmid with Hsp82 heterodimer construct.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiB (HSP82 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   GPD1

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHOHO2_Hsp82viiA

    Plasmid

    #61708

    Purpose

    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

    Depositor

    Dan Bolon

    Article

    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

    Insert

    Hsp82viiA (HSP82 Budding Yeast)
    

    Use

    Yeast integration vector targeting inserts to the…

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Heteromeric coiled coil inserted after amino acid…

    Promoter

    TEF1

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV106-pTDH3-mCherry-10xSumo-6xSIM

    Plasmid

    #116928

    Purpose

    Yeast integrative vector for expression of the SUMO-SIM complex

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10xSUMO-6xSIM
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ube2d1

    Plasmid

    #31409

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2D1 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. PTEF1-yEGFPCLN2PEST-pRS406

    Plasmid

    #64406

    Purpose

    TEF promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    TEF1 promoter (TEF1 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p403 – TDH3-MTS-mCherry-GFP1-10-His

    Plasmid

    #177730

    Purpose

    mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    mCherry-GFP1-10
    

    Use

    Tags

    Mitochondrial Targeting Sequence

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRP2365

    Plasmid

    #127575

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAR-Ec318

    Plasmid

    #130871

    Purpose

    A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKanB alpha

    Plasmid

    #223524

    Purpose

    Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. YIplac211-SEC7-mCherry2Bx6

    Plasmid

    #105267

    Purpose

    Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Sec7 portion (SEC7 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ube2b

    Plasmid

    #31408

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2B Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pG2

    Plasmid

    #162603

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge