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Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_MFE1_hrGFP
Plasmid#84617PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(1-93).ER.VP16
Plasmid#108216PurposeYeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domainDepositorInsertGal4 DBD-ERalpha HBD-VP16
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH1Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ytdTomato
Plasmid#118456PurposeOverexpression of yeast codon-optimized tdTomatoDepositorInsertytdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4599
Plasmid#129660PurposeNATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-HIS3MX6
Plasmid#20762DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pRS306-pHIS3-2xGFP-tURA3
Plasmid#116936PurposeYeast integrative vector for the expression of 2xGFPDepositorInsert2xGFP
UseTagsExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtnluc
Plasmid#184339PurposeTransformation of mitochondrial DNA in yeastDepositorInsertnanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-L
Plasmid#222847PurposeYeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarlet3-T2A-mTurquoise2
Plasmid#219848Purposeequimolar expression of mScarlet3 and mTurquoise2 in budding yeastDepositorInsertmScarlet3-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRG706_lexO-SbfI_LexA-TF_LEU2MX
Plasmid#154818PurposeInducible expression of SbfI endonuclease in S. cerevisiaeDepositorInsertsSbfI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-Dcr1
Plasmid#22307DepositorInsertDCR1
Use2micronTagsExpressionYeastMutationPromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3-GFP-Atg8
Plasmid#69194PurposeSingle copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertGFP-Atg8
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-nLuc
Plasmid#199755PurposeControl construct of elongation reporter to quantify elongation of YFPDepositorInsertNanoluc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Phleomycin
Plasmid#188915PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Phleomycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only