8,057 results

1. pCE41

   Plasmid

   #174433

   Purpose

   C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2015-N iFAST

   Plasmid

   #209415

   Purpose

   iFAST expression plasmid using MET3p

   Depositor

   Patrick Van Dijck

   Article

   Devos et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   iFAST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   MET3

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-HDEL

   Plasmid

   #129098

   Purpose

   For labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDEL

   Depositor

   Wei-Lih Lee

   Article

   Omer et al Elife. 2018 Aug 7;7. pii: 36745. doi: 10

   Insert

   TEF1p-SS-3xGlyAla-GFP-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG416GAL-ccdB

   Plasmid

   #14147

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM64

   Plasmid

   #133655

   Purpose

   Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-COX4-DuDre (COX4 Budding Yeast)
   

   Use

   Tags

   DuDre

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LT1_33_pTEFmut8-mCherry

   Plasmid

   #177706

   Purpose

   Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   pTEFmut8-mCherry
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG426GPD-ccdB-YFP-yeastSMASh

   Plasmid

   #68855

   Purpose

   Expresses SMASh tag fused to YFP at C terminus in yeast

   Depositor

   Michael Lin

   Article

   Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

   Insert

   YFP-yeastSMASh
   

   Use

   Tags

   SMASh tag

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV0757

   Plasmid

   #133509

   Purpose

   Expression of 3GFP tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDR-GW FLII12Pglu-700μδ6

    Plasmid

    #28002

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLII12Pglu-700μδ6
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-ymNeongreen-CaURA3

    Plasmid

    #125703

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059  pFA6a-link-ymNeongreen-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ_P-GAP-eGFP

    Plasmid

    #126717

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pABY-T3

    Plasmid

    #201766

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Linked Free ClLIS1 (M)

    Plasmid

    #182487

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS(M)-LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPL5608_TEF1*-Cre_TRP1

    Plasmid

    #60931

    Purpose

    Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    Mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAS9id

    Plasmid

    #141251

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCT2

    Plasmid

    #130970

    Purpose

    HyPr plasmid with natMX marker

    Depositor

    Chris Todd Hittinger

    Article

    Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

    Insert

    empty backbone
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHMK34

    Plasmid

    #130971

    Purpose

    HyPr plasmid with hphMX marker

    Depositor

    Chris Todd Hittinger

    Article

    Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

    Insert

    empty backbone
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pABY-T4

    Plasmid

    #201767

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge