8,016 results

1. pBJ1807 pRS415-COF1RFP

   Plasmid

   #37102

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Promoter

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

2. NAT-TET-OFF -MET - 3xHA

   Plasmid

   #182526

   Purpose

   Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Use

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-hphMX6-Purg1-3FLAG

   Plasmid

   #19356

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   urg1 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOP459

   Plasmid

   #85933

   Purpose

   Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

   Depositor

   Timothy Lu

   Article

   Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

   Insert

   Anti-Ebola 2G4 monoclonal antibody
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PhyB-mCherry-Htb2

   Plasmid

   #51569

   Purpose

   Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   HTB2 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPS1372

   Plasmid

   #1353

   Depositor

   Pamela Silver

   Article

   Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

   Insert

   pADH-NLS-NES-GFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pAG303GAL-ccdB-EGFP

   Plasmid

   #14181

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKT0176

   Plasmid

   #8743

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   tdimer2
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR FLIP43 GPS1-NR

   Plasmid

   #101085

   Purpose

   GA biosensor expression plasmid

   Depositor

   Alexander Jones, Wolf Frommer

   Article

   Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

   Insert

   GPS1-NR
   

   Use

   Tags

   6HIS and cMyc

   Expression

   Yeast

   Mutation

   AtGAI E51K E54R, AtGID1C S114A F115A

   Promoter

   pPMA1

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-link-yomWasabi-Kan

    Plasmid

    #44945

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomWasabi
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMJA104

    Plasmid

    #128520

    Purpose

    Expresses amyloid human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme I56T (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GAL 72Q+ProGFPp416

    Plasmid

    #15582

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q + Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pGPD-luxAB(HIS)

    Plasmid

    #1106

    Depositor

    Susan Lindquist

    Article

    Parsell et al Nature 1994 Dec 1;372(6505):475-8.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pDBD-M

    Plasmid

    #50572

    Purpose

    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

    Depositor

    Alexander Varshavsky

    Article

    Turner et al Science. 2000 Sep 22;289(5487):2117-20.

    Insert

    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pKT0140

    Plasmid

    #8732

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECitrine
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with S65G, V68L, Q69M, S72A, T203Y

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nuc-yHS1

    Plasmid

    #159166

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. LD201

    Plasmid

    #69178

    Purpose

    Integration of 2GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg1-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LD202

    Plasmid

    #69179

    Purpose

    Integration of 2GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg2-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-yoLSS-mKate2-CaURA3

    Plasmid

    #44886

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYTSG

    Plasmid

    #159419

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Use

    Tags

    polyhistidine (poly-His)

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 426Gal-FUS-RRM Mutant-YFP

    Plasmid

    #29608

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS RRM mutant (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    F305L, F341L, F359L and F368L

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

23. BS-Leu2

    Plasmid

    #69200

    Purpose

    complementation of yeast auxotrophic marker LEU2

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    LEU2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTH671-SUP35-H348Q

    Plasmid

    #29732

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H348Q mutant gene of SUP35 including genomic sequ…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ZJOM2

    Plasmid

    #133633

    Purpose

    Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VRG4-GFP (VRG4 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 3019_pETcon-SARS-CoV-2-RBD_E484K

    Plasmid

    #184404

    Purpose

    yeast surface display of the SARS-CoV-2 Eta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    E484K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJJB170

    Plasmid

    #158600

    Purpose

    Transcription factors ADR1c, PIP2c, OAF1c

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Inserts

    ADR1c
    PIP2c
    OAF1c
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-link-yomEos2-CaURA3

    Plasmid

    #44887

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEos2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCUP1-GR

    Plasmid

    #1212

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    glucocorticoid receptor (Nr3c1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pH3FS-GAL1

    Plasmid

    #85782

    Purpose

    N-ICE plasmid pH3FS-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pILGFP5EF3

    Plasmid

    #185881

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFA6a-3GFP-kanMX6

    Plasmid

    #105148

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, David Kovar, Thomas Pollard

    Article

    Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

    Insert

    3GFP-kanMX6
    

    Use

    Tags

    3GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS424-TUB2-Cterminal6xHis

    Plasmid

    #60386

    Purpose

    Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    6xHIS

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

34. ZJOM141

    Plasmid

    #133669

    Purpose

    Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX9-mTagBFP2 (COX9 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMLB10

    Plasmid

    #177273

    Purpose

    GAL1p:T7RNAP(F11L;T613A):CYC1t

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Insert

    T7RNAP(F11L;T613A)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    F11L T613A

    Promoter

    GAL1

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-6xGLY-Myc-HIS3MX6

    Plasmid

    #20768

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRMH120

    Plasmid

    #40593

    Purpose

    Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression

    Depositor

    Chandra Tucker

    Article

    Hughes et al Methods. 2012 Aug 15.

    Inserts

    CRY2 (CRY2 Mustard Weed)
    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and TEF

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pK3F

    Plasmid

    #85770

    Purpose

    N-ICE plasmid pK3F

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p426-Cup1p-FUS-FusionRed

    Plasmid

    #188393

    Purpose

    Cu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cells

    Depositor

    Michihiko Kataoka

    Article

    Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FusionRed

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJZC506

    Plasmid

    #62281

    Purpose

    COM-VP64 fusion

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    COM-VP64
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Promoter

    pAdh

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge