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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mECitrine

Plasmid

#105151

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

kanMX6-P3nmt1-mECitrine

Tags

3nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

3nmt1

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mECitrine

Plasmid

#105153

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

Insert

kanMX6-P81nmt1-mECitrine

Tags

81nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

81nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGWKS4

Plasmid

#139411

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mCherry

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pFA6a-link-mCherry-SpHis5

Plasmid

#44643

Purpose

Yeast tagging vector to create gene fusion with mCherry with SpHis5 selection

Depositor

Kurt Thorn

Insert

mCherry

Expression

Yeast

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCfb12620

Plasmid

#219928

Purpose

PrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TPI

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKmK.T3

Plasmid

#125055

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

KMXK_A3020 terminator

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPL5606_TEF1*-Cre_ADE2

Plasmid

#60932

Purpose

Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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P415 ymScarletI3

Plasmid

#224111

Purpose

Expressing ymScarletI3 in yeast

Depositor

Bas Teusink

Insert

ymScarletI3

Expression

Yeast

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKmK.I2R

Plasmid

#125064

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-Purg1-3FLAG

Plasmid

#19356

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

urg1 promoter

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pKmK.I2L

Plasmid

#125031

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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p426*-SpSgH

Plasmid

#136993

Purpose

Helper plasmid for SpCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymScarletI3_T2A_mTurquoise2

Plasmid

#219829

Purpose

equimolar expression of ymScarletI3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymScarletI3_T2A_mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTEF1_MS2

Plasmid

#220625

Purpose

Source for TEF1 MS2 coat fusion protein fusion

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-no_intron

Plasmid

#127597

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

1xFLAG-MCP expression cassette

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pV1090

Plasmid

#111427

Purpose

gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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