8,086 results

1. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

   Plasmid

   #51690

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   hisG-URA3-hisG (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNB768

   Plasmid

   #60139

   Purpose

   Green click beetle luciferase from Promega driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   CBG99
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHpgRNA13

   Plasmid

   #160127

   Purpose

   To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

   Depositor

   Yongjin Zhou

   Article

   Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

   Insert

   PTEF1-HH-gRNA-ADE2- HDV-TAMO
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry

   Plasmid

   #203656

   Purpose

   Yeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Tags

   yEmCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

   Plasmid

   #58432

   Purpose

   Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)1-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pROS10

   Plasmid

   #107924

   Purpose

   URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV0761

   Plasmid

   #133494

   Purpose

   Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ZJOM44

   Plasmid

   #133653

   Purpose

   Integration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SNF7-mCherry (SNF7 Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PP78

    Plasmid

    #78948

    Purpose

    Supplemental Figure 3. Integration Site 5.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    Integration Site 5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPTK010-3a-Glucoamylase-αMFΔ

    Plasmid

    #84969

    Purpose

    Type 3a, Glucoamylase followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Glucoamylase followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB6371

    Plasmid

    #106153

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-G9-5FLAG-hphMX6

    Plasmid

    #49185

    Purpose

    genomic G9-5FLAG epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    G9-5FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDRF1-GW ymVenus

    Plasmid

    #118454

    Purpose

    Overexpression of yeast codon-optimized mVenus

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKL05

    Plasmid

    #104429

    Purpose

    super Ecliptic pHluorin in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    Super Ecliptic pHluorin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLexA

    Plasmid

    #11342

    Depositor

    Guy Caldwell

    Type

    Empty backbone

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pSAC200

    Plasmid

    #187911

    Purpose

    psac200-crispey-bar-vector

    Depositor

    Hunter Fraser

    Article

    Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSAC212

    Plasmid

    #187912

    Purpose

    psac212-crispey-bar-ade2KO

    Depositor

    Hunter Fraser

    Article

    Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

    Insert

    ade2 KO CRISPEY cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRR-GR-5Z

    Plasmid

    #23060

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    GR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only