8,404 results
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Plasmid#130972PurposeHyPr plasmid with ble markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM40
Plasmid#133650PurposeIntegration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZaa-STE2-cohis
Plasmid#133433PurposeYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
ExpressionYeastMutationC59S/C252A, Codon optimized for P. pastoris, and …Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
ExpressionYeastPromotermin pCYC1Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8788
Plasmid#126922PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1DepositorInsertXdCrtI and HMG1
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0752
Plasmid#133486PurposeExpression in fission yeast from promoter pnmt41 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAN200
Plasmid#129203PurposeyTRAP sensor of [PSI+], detects aggregation of Sup35DepositorInsertSup35NM (SUP35 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationN terminal and middle domainsPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV106-pTDH3-mCherry-10xSumo-6xSIM
Plasmid#116928PurposeYeast integrative vector for expression of the SUMO-SIM complexDepositorInsert10xSUMO-6xSIM
TagsmCherryExpressionYeastPromoterTDH3Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C3
Plasmid#125061PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN6
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C2
Plasmid#125060PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN5
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS2247
Plasmid#123026PurposeTet promoter (TetO(x7)-pLEU2m)DepositorInsertpTet [2]
ExpressionYeastAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymYPET
Plasmid#118455PurposeOverexpression of ymYPETDepositorInsertymYPET
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-amdSYM-mak10TR
Plasmid#75433PurposeGenerates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiaeDepositorInsertsMAK10TR
ApR
amdSYM
ExpressionYeastMutationN-terminal 740 bp of MAK10 ORFPromoterNative promoter and PTEFAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1081
Plasmid#111425PurposeSolo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1DepositorInsertCaCas9/sgADE-NT2
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec31-yoHalo
Plasmid#115423PurposeExpress Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec31-yoHalo
TagsyoHaloTagExpressionYeastPromoterEndogenousAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1539
Plasmid#111434PurposeGenedrive entry vector for Candida albicansDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AC
Plasmid#108686PurposePlasmid for assembly of donor DNA templates consisting of two expression unitsDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScDD2_ERdd
Plasmid#109047Purposedestabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).DepositorInsertER50 (ESR1 H. sapiens gene with yeast enhanced codon)
Tagsyeast enhanced GFP with 8 amino acids TSGRGEGQ li…ExpressionYeastMutation3 mutations : E380G, R434G, N532SPromoterSOD1Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG/ER(G)
Plasmid#108219PurposeYeast expression vector for human estrogen receptor alphaDepositorAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only