8,086 results

1. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

   Plasmid

   #173452

   Purpose

   Contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

   Insert

   mCherry-sfGFP-ADH1ter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH761-CEN-mCherry_v4

   Plasmid

   #40599

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   mCherry ORF partially codon optimised for yeast e…

   Promoter

   GPD (TDH3)

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFP92

   Plasmid

   #83549

   Purpose

   Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TDH3 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCA/GAL4(1-93).ER.VP16

   Plasmid

   #108216

   Purpose

   Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

   Depositor

   Didier Picard

   Article

   Louvion et al Gene 1993 Sep 6;131(1):129-34.

   Insert

   Gal4 DBD-ERalpha HBD-VP16
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERalpha HBD has G400V mutation

   Promoter

   ADH1

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPObooster

   Plasmid

   #216160

   Purpose

   Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

   Depositor

   Grant Brown

   Article

   Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

   Inserts

   MKT1(30G) (MKT1 Budding Yeast)
   RME1(ins-308A) (RME1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   MKT1 gene containing an aspartic acid to glycine …

   Promoter

   endogenous promoter

   Available Since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBHM2403

   Plasmid

   #171687

   Purpose

   pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   CnoCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gal:mCherry-CRY2-uTEV1Δ(220-242)

   Plasmid

   #135452

   Purpose

   Yeast expression of uTEV1Δ fused to CRY2 and mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-CRY2-TEV1Δ(220-242)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Gal

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yoEGFP-CaURA3

    Plasmid

    #44872

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLSB-KAN

    Plasmid

    #166700

    Purpose

    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    adh15 promoter and tRNA Ser promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRF1-GW yoeGFP-T2A-mCherry

    Plasmid

    #118446

    Purpose

    Produces equimolar expression of yeast codon-optimized eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yoeGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTPGI_dCas9

    Plasmid

    #64279

    Purpose

    encodes yeast-optimized dCas9 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    Yeast-optimized dCas9
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    D10A & H840A relative to S. pyogenes Cas9

    Promoter

    pTPGI

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-FKBP(M)x4

    Plasmid

    #85003

    Purpose

    Expresses EGFP-FKBP(M)x4 in yeast

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    EGFP-FKBP(M)x4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGal4BD-CRY2

    Plasmid

    #28243

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBHM2329

    Plasmid

    #171686

    Purpose

    pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    C. neoformans U6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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