7,935 results
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Plasmid#17190DepositorInsertXdsh (dvl2.L Frog)
UseTagsGal4-DBDExpressionYeastMutationDsh bp 343-1191PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDR-AmTrac-LS
Plasmid#47767Purposeexpresses AmTrac-LS in yeastDepositorInsertAmTrac-LS
UseTagsmcpGFPExpressionYeastMutationPromoterPMAAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ccdB-DsRed
Plasmid#14368DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS316-Cwc25
Plasmid#111343PurposeCwc25 in pRS316(Ura) vectorDepositorInsertCWC25
UseTagsnoneExpressionYeastMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH751-CEN-RLuc/min53maxCFLuc
Plasmid#38217DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe first 53 codons of the original FLuc gene wer…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG415-ADH1p-HA-rap1-K9R
Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3NR
Plasmid#84533PurposeThis plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,117,118)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3CR
Plasmid#84534PurposeThis plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(462,463,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K9R
Plasmid#84535PurposeThis plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK651RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB-ECFP
Plasmid#14175DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-HA-TMSec66-SrtA (pKS105)
Plasmid#50033PurposeGalactose-inducible expression of ER-membrane S. pyogenes SrtA in yeastDepositorInsertSrtA(strep)
UseTagsHAExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL10 - Brr2 del422 WT
Plasmid#90260Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acidsPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC861
Plasmid#20391DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiY (see schematic) linker: ekPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ECFP-ccdB
Plasmid#14294DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-EYFP
Plasmid#14222DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pAG303GPD-EYFP-ccdB
Plasmid#14326DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG305GPD-EYFP-ccdB
Plasmid#14330DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YEplac181-DST1
Plasmid#8624DepositorInsertDST1 (DST1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
p41Neo 1-F GW
Plasmid#58545PurposeCEN vector with G418 resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCALex
Plasmid#17265DepositorTypeEmpty backboneUseTagsLexAExpressionYeastMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-DsRed
Plasmid#14355DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Synthetic, Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPS1889
Plasmid#1361DepositorInsertNLS-ECFP-EYFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
p2HGAL/CGR/CYC
Plasmid#1218DepositorInsertCGR (Nr3c1 Rat)
UseTagsExpressionYeastMutationpRS423 with GAL promoter and CYC terminator gene…PromoterAvailable sinceJuly 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-NYFP
Plasmid#99557PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 N-rich region (AA1-327) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-TAP
Plasmid#14270DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_AH
Plasmid#98556Purposeempty BB3 for insertion and overexpression of 7 genes in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB-ECFP
Plasmid#14169DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHK
Plasmid#64187Purposeharbours marker genes HIS3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAH3
Plasmid#41827PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-C67Y
Plasmid#10982DepositorInsertrpb1-C67Y (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 200 G to A in the RPB1 ORF resulting i…PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-C70Y
Plasmid#10983DepositorInsertrpb1-C70Y (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 209 G to A in the RPB1 ORF resulting i…PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-H80Y
Plasmid#10984DepositorInsertrpb1-H80Y (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 238 C to T in the RPB1 ORF resulting i…PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Lys13Arg
Plasmid#50722Purposeexpression of the human TPI Lys13Arg allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationchanged Lysine 13 to ArgeninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Thr
Plasmid#50721Purposeexpression of the human TPI Ile170Thr allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ThreoninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB4780
Plasmid#106143PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-NAT
Plasmid#80588PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterNative WHI2 promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH743-CEN-RLuc/slowstaCFLuc
Plasmid#38223DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH742-CEN-RLuc/slowminCFLuc
Plasmid#38222DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJT3
Plasmid#98304PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-ccdB-EYFP
Plasmid#14207DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-81nmt1
Plasmid#105164PurposeYeast gene targetingDepositorInserthphMX6-81nmt1
UseTags81nmt1ExpressionBacterial and YeastMutationPromoter81nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-41nmt1
Plasmid#105163PurposeYeast gene targetingDepositorInserthphMX6-41nmt1
UseTags41nmt1ExpressionBacterial and YeastMutationPromoter41nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pXP711
Plasmid#46053DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXP712
Plasmid#46054DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPGK1Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only