8,030 results

1. 426Gal-FUS-1-413aa-YFP

   Plasmid

   #29599

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS-1-413aa (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Contains FUS aa# 1-413

   Promoter

   Available since

   July 18, 2011

   Availability

   Academic Institutions and Nonprofits only

2. 416Gal-FUS-R514G-YFP

   Plasmid

   #29616

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R514G

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. MET FLAG103QDProCFPp303

   Plasmid

   #15591

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 103Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. yCp50-PRP2

   Plasmid

   #111304

   Purpose

   Prp2 in yCp50 plasmid

   Depositor

   Christine Guthrie

   Article

   Noble et al Genetics. 1996 May;143(1):67-80.

   Insert

   PRP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS306Sup35R1

   Plasmid

   #17715

   Depositor

   Susan Lindquist

   Article

   Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

   Insert

   pRS306Sup35R1 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   An pRS306 based integrating plasmid designed for …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCK011

   Plasmid

   #105343

   Purpose

   Yeast two hybrid

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCK012

   Plasmid

   #105344

   Purpose

   Yeast two hybrid

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MS2-ecDHFR

   Plasmid

   #65859

   Purpose

   Expresses RNA-binding protein MS2 with eDHFR fusion tag

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

   Inserts

   MS2
   E. coli Dihydrofolate Reductase (folA )
   

   Use

   Tags

   HA and NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMGF173

   Plasmid

   #96999

   Purpose

   NLS-GFP-lacZ leu1 insertion vector

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   lacZ
   

   Use

   Tags

   NLS-GFP

   Expression

   Yeast

   Mutation

   Promoter

   Pnmt1

   Available since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWZ414-F55

    Plasmid

    #23079

    Depositor

    Sharon Dent

    Article

    Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Histone H3 changed Lysine 9 to Arginine, H3 K9R …

    Promoter

    Available since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pWZ414-F31

    Plasmid

    #23067

    Depositor

    Sharon Dent

    Article

    Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Histone H3 changed lysine 14 to Glutamine K14Q

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

12. HC080

    Plasmid

    #59545

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag

    Depositor

    Daniel Klionsky

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. p413TEF-HST2

    Plasmid

    #51745

    Purpose

    overexpression of HST2 in S. cerevisiae, HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    HST2 (HST2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AausFP1-T2A-mCherry pdrf1-GW

    Plasmid

    #168071

    Purpose

    Produces equimolar expression of AausFP1 and mCherry for use in yeast

    Depositor

    Bas Teusink

    Insert

    AausFP1-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHCA/GAL4(848).ER

    Plasmid

    #108215

    Purpose

    Yeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor a

    Depositor

    Didier Picard

    Insert

    Gal4-ERalpha HBD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERalpha HBD has G400V mutation

    Promoter

    ADH0

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDN-NG1Tt

    Plasmid

    #44523

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1 (wt) promoter (GAL1 Budding Yeast)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGal1 (wt)

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMJA053

    Plasmid

    #128515

    Purpose

    Expresses SFA8 in Pichia

    Depositor

    Marcos Alcocer

    Article

    Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

    Insert

    SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUDR217

    Plasmid

    #113872

    Purpose

    expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA9-MPH2-3 sgRNA10-MAL11
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316-RGR-GFP-mm

    Plasmid

    #51059

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP-GM2

    Plasmid

    #64740

    Purpose

    Yeast expression plasmid

    Depositor

    Jens Nielsen

    Article

    Chen et al FEMS Yeast Res. 2012 Aug;12(5):598-607.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pBCSK_HIS3_II

    Plasmid

    #109059

    Purpose

    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXRU3

    Plasmid

    #63152

    Purpose

    Ura3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LHP585 - Ub-CYC1

    Plasmid

    #32168

    Depositor

    Linda Hicke

    Article

    Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

    Insert

    uba1 yeast (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pXP114

    Plasmid

    #26812

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVEG17*

    Plasmid

    #111856

    Purpose

    Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

    Depositor

    Nikhil Nair

    Article

    Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

    Insert

    GAL1p-GAL3-Syn4.1-TEFt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    D68N, V69M, A109V, I271L

    Promoter

    GAL1p

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CUP Puf2 YFP p316

    Plasmid

    #15595

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    PUF2 (PUF2 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

27. Gal tr-lux

    Plasmid

    #1330

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

    Insert

    Bacterial luciferase-V.h.CTP5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

28. pAG423GAL-ccdB-DsRed

    Plasmid

    #14365

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNX3a-PK3

    Plasmid

    #78346

    Purpose

    fission yeast epitope tagging

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xPK
    kanMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRG711_lexO-AscI_LexA-TF_HIS3MX

    Plasmid

    #154820

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pICE MET17

    Plasmid

    #122880

    Purpose

    MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFA1

    Plasmid

    #46789

    Purpose

    To express Fun30 in trans in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    Promoter

    PGAL1

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pAG423GAL-EYFP-ccdB

    Plasmid

    #14341

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGPDluxAB(Ura)

    Plasmid

    #1119

    Depositor

    Susan Lindquist

    Article

    Singer et al Mol Cell 1998 Apr;1(5):639-48.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

35. pUSti1

    Plasmid

    #1335

    Depositor

    Susan Lindquist

    Article

    Chang et al Mol Cell Biol 1997 Jan;17(1):318-25.

    Insert

    Sti1 (STI1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

36. LifeAct-mScarletI pDRF1-GW

    Plasmid

    #168085

    Purpose

    Expresses Lifeact-mScarletI to visualize actin in yeast

    Depositor

    Bas Teusink

    Insert

    LifeAct-mScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDDGFP-Leu2d_GgPCFT

    Plasmid

    #165414

    Purpose

    expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT (SLC46A1 Synthetic, Chicken)
    

    Use

    Tags

    GFP-His8

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCSN069

    Plasmid

    #166728

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB3053(gRNA X-2, XI-5, XII-4)

    Plasmid

    #73295

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge