Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

Yeast Expression

7,935 results

Showing: 4301 - 4350 of 7935 results

  1. pAG426-GAL-3xFLAG-PolyC

    Plasmid
    #181729
    Purpose
    Galactose inducible expression of 3xFLAG-PolyC
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG-PolyC
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    181729-plasmid-map-sequence-id-468511

    Enlarge

  2. pAP-U2-4

    Plasmid
    #236475
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    scACT1p-3xmCherry apTUB1p-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236475-plasmid-map-sequence-id-468488

    Enlarge

  3. pAP-U2-2

    Plasmid
    #236473
    Purpose
    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.
    Depositor
    Daniel Lew
    Article
    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.
    Insert
    spH2Bp-3xmCherry scACT1p-3xGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for A. pullulans
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236473-plasmid-map-sequence-id-468487

    Enlarge

  22. ACT1-CUP1 9bp dist

    Plasmid
    #234131
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234131-plasmid-map-sequence-id-466522

    Enlarge

  23. ACT1-CUP1 12bp dist

    Plasmid
    #234130
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234130-plasmid-map-sequence-id-466519

    Enlarge

  24. ACT1-CUP1 27bp dist

    Plasmid
    #234129
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234129-plasmid-map-sequence-id-466539

    Enlarge

  25. ACT1-CUP1 15bp dist

    Plasmid
    #234128
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234128-plasmid-map-sequence-id-466529

    Enlarge

  26. ACT1-CUP1 42bp dist

    Plasmid
    #234127
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234127-plasmid-map-sequence-id-466531

    Enlarge

  27. ACT1-CUP1 50bp dist

    Plasmid
    #234112
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234112-plasmid-map-sequence-id-466054

    Enlarge

  28. ACT1-CUP1 46bp dist

    Plasmid
    #234111
    Purpose
    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone
    Depositor
    Aaron Hoskins
    Insert
    ACT1-CUP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Intron truncation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234111-plasmid-map-sequence-id-466051

    Enlarge

  42. pCfb13198

    Plasmid
    #219873
    Purpose
    The base plasmid of TUNEYALI forTF08
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    219873-plasmid-map-sequence-id-467444

    Enlarge

  43. pCfB2188-PrARG8-GFP::URA3

    Plasmid
    #216512
    Purpose
    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrARG8-yeGFP
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ARG8
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216512-plasmid-map-sequence-id-429384

    Enlarge

  44. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid
    #216513
    Purpose
    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrGnp1-mKate2
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GNP1
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216513-plasmid-map-sequence-id-429383

    Enlarge

  46. pBHM2643

    Plasmid
    #229527
    Purpose
    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mAID_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    229527-plasmid-map-sequence-id-459548

    Enlarge

Showing: 4301 - 4350 of 7935 results