7,933 results

1. pHpgRNA13

   Plasmid

   #160127

   Purpose

   To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

   Depositor

   Yongjin Zhou

   Article

   Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

   Insert

   PTEF1-HH-gRNA-ADE2- HDV-TAMO
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. bRA90

   Plasmid

   #100951

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-SKII-3XHA-HALO-NAT

   Plasmid

   #188931

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-NAT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYES2.1-pomMet8

   Plasmid

   #107449

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met8
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEV-G2-Km

   Plasmid

   #46815

   Purpose

   TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

   Depositor

   Claudia Vickers, Lars Nielsen

   Article

   Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

   Type

   Empty backbone

   Use

   Tags

   FLAG and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 and PGK1

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-ymYPET-URA3

   Plasmid

   #168056

   Purpose

   Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   ymYPET

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBridge-tyrosinase.σ2

   Plasmid

   #197415

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-2 σ2
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   encodes R42G substitution, contains silent substi…

   Promoter

   ADH1 and MET25

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-6xGLY-V5-kanMX6

   Plasmid

   #20780

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-yomCherry-SpHis5

   Plasmid

   #44841

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mCherry with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDS190

    Plasmid

    #34977

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge