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Plasmid#89960PurposeContains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.DepositorInsertempty gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-NT
Plasmid#37818DepositorInsertPromotorless gfp reporter gene
UseTagsExpressionMutationPromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pPROBE-NT'
Plasmid#37819DepositorInsertPromotorless gfp reporter gene
UseTagsExpressionMutationPromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCIS.MTRIA-NTS
Plasmid#184614PurposeFor expression of a fluorescent sensor for neurotensin in mammalian cellsDepositorInsertMTRIA-NTS
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGGSx4 NT
Plasmid#165810PurposeFlexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 partsDepositorInsertGGSx4 NT
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS
Plasmid#26019DepositorInsertVinculin tension sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
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NTSR2-Tango
Plasmid#66458PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertNTSR2 (NTSR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
NTSR1-DuET
Plasmid#213356PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNTSR1 (NTSR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NTSR1-Tango
Plasmid#66457PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertNTSR1 (NTSR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS
Plasmid#111830PurposeVinculin tension sensor in lentiviral expression vectorDepositorInsertVinculinTS (VCL Chicken)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD19_STARR-seq_plants
Plasmid#117379PurposeSTARR-seq vector with 35s core promoter for transient expression in plant protoplastsDepositorTypeEmpty backboneUseTagsGFP derived from pMDC107ExpressionPlantMutationPromoter35s core promoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tailless VinculinTS
Plasmid#26020DepositorInserttailless vinculinTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I
Plasmid#111827PurposeVinculin tension sensor with point mutation affecting ability to bind talinDepositorInsertVinculinTS (A50I)
UseTagsExpressionMammalianMutationchanged alanine 50 to isoleucinePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-I997A
Plasmid#111828PurposeVinculin tension sensor with point mutation affecting ability to bind actinDepositorInsertVinculinTS (I997A)
UseTagsExpressionMammalianMutationchanged isoleucine 997 to alaninePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationfull-lengthPromotertac promoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_NTS1.0
Plasmid#208670PurposeExpresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cellsDepositorInsertGPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1R2B2
Plasmid#160882PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXR1AtR2B2NtS1
Plasmid#160879PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: pBAD, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2B2
Plasmid#160880PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS170-enNTS1
Plasmid#199152PurposeJZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)DepositorInsertenNTS1 (Ntsr1 Rat)
UseTags10xHis tag, Avi tag, MBP, and muGFPExpressionBacterialMutationV208M, C273S, C391S and C393S (over NTS1-B5)PromoterLac/T5Available sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-C
Plasmid#128417PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationINTS3 C-terminal region (514-1042 aa)PromoterTac promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS5R1AtR2NtB2
Plasmid#160884PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S5
UseTagsExpressionBacterialMutationPromoterrbcS-S5: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS2R1AtR2NtB2
Plasmid#160883PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S2, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S2: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 IntS12
Plasmid#210521PurposeExpresses GST-tagged Drosophila IntS12DepositorInsertIntS12 (IntS12 Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only