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Plasmid#127602Purposelow-copy URA-selectable plasmid, ACT1 intron inserted into URA3DepositorInsertACT1 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Fyv6
Plasmid#234141PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Syf1
Plasmid#234113PurposeSyf1 region PCR, restriction digest and ligation into pRS416 backboneDepositorInsertSyf1
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG
Plasmid#158145PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ECM33_intron
Plasmid#127596Purposelow-copy URA-selectable plasmid, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-RPL27B_intron
Plasmid#127604Purposelow-copy URA-selectable plasmid, RPL27B intron inserted into URA3DepositorInsertRPL27B intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-APS3_intron
Plasmid#127605Purposelow-copy URA-selectable plasmid, APS3 intron inserted into URA3DepositorInsertAPS3 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HNT2_intron
Plasmid#127603Purposelow-copy URA-selectable plasmid, HNT2 intron inserted into URA3DepositorInsertHNT2 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Hsh155 WT
Plasmid#87219PurposePlasmid used to maintain viability after deletion of genomic copy of HSH155.DepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS416Gal TDP43 WT YFP
Plasmid#27447DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 GAL1pro-YFP
Plasmid#224875PurposeLow copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)DepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterGAL1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 WT
Plasmid#27458DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416gT-GalL-Cas9
Plasmid#79903PurposepRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expressionDepositorInsertsCas9
guide RNA (gRNA)
Tetracycline Repressor
UseCRISPRTagsExpressionYeastMutationPromoterGPM1 promoter, GalL, and RPR1 promoter with TetO …Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6L1TAG
Plasmid#158148PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertAga1p-FAPB2.3.6
UseTagsExpressionYeastMutationCloned in a TAG codon at the first position of th…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ1808 pRS416-COF1RFP
Plasmid#37103DepositorInsertCOF1-RFP
UseTagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …PromoterAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1
Plasmid#18684DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG-altTAG
Plasmid#158146PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal Q331K YFP
Plasmid#27450DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationGlutamine 331 mutated to Lysine (Q331K)PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr (RB3578)
Plasmid#69099PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3prDepositorInsertsyZ3EV cassette
Z3 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Osh1
Plasmid#58835PurposeExpresses GFP-Tagged Osh1 in yeastDepositorUseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal M337V YFP
Plasmid#27449DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationMethionine 337 mutated to Valine (M337V)PromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Gal4-dCas9-VP64
Plasmid#71128PurposeThis plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.DepositorInsertGal4-dCas9-VP64
UseTagsNLS n-terminal, N terminal Gal4 Activator domain,…ExpressionYeastMutationPromoterTef1Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal G294A YFP
Plasmid#27448DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 M337V
Plasmid#27460DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationMethionine 337 mutated to Valine (M337V)PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 Q331K
Plasmid#27461DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlutamine 331 mutated to Lysine (Q331K)PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Osh3
Plasmid#58839PurposeExpresses GFP-Tagged Osh3 in yeastDepositorInsertOsh3p (OSH3 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1-YFP
Plasmid#18685DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 G294A
Plasmid#27459DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-3P-ECM33_intron
Plasmid#127601Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 3′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5M-ECM33_intron
Plasmid#127600Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-5P-ECM33_intron
Plasmid#127599Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3DepositorInsertECM33 intron with MS2 hairpins inserted near 5′ end of intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-ECM33_intron
Plasmid#127598Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-no_intron
Plasmid#127597Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoterDepositorInsert1xFLAG-MCP expression cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 L94A
Plasmid#18691DepositorInsertRnq1 L94A
UseTagsExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Opi1 + terminator
Plasmid#58836PurposeExpresses GFP-Tagged Opi1 + Terminator in yeastDepositorInsertOpi1p (OPI1 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS416 ATG5-E141D-HA-ATG12-ATG16
Plasmid#166851PurposeExpresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.DepositorUseTagsHA-ATG12ExpressionYeastMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 1-153-YFP
Plasmid#18690DepositorInsertRnq1 1-153
UseTagsYFPExpressionYeastMutationNonPrion domain of Rnq1PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only