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We narrowed to 2,817 results for: ALC

Showing: 1 - 40 of 2817 results

  4. pmalc4x_E323K_TEV

    Plasmid
    #47699
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed glutamic acid 323 to lysine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47699-plasmid-map-sequence-id-68104

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  6. pmalc4x_G252R_TEV

    Plasmid
    #47704
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with G252R mutation (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Glycine 252 Arginine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47704-plasmid-map-sequence-id-68124

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  7. pmalc4x_K398R_TEV

    Plasmid
    #47701
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with K398R mutation (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Lysine 398 to Arginine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47701-plasmid-map-sequence-id-68116

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  9. CALCRb-Tango

    Plasmid
    #66233
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CALCRb (CALCRL Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. CALCRL-Tango

    Plasmid
    #66234
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CALCRL (CALCRL Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66234-plasmid-map-sequence-id-347881

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  14. CALCRL-DuET

    Plasmid
    #213194
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CALCRL (CALCRL Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213194-plasmid-map-sequence-id-431751

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  18. CALCRb-DuET

    Plasmid
    #213193
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CALCRb (CALCRL Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213193-plasmid-map-sequence-id-430758

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  19. pET28a MDH_PFALCI

    Plasmid
    #204235
    Purpose
    Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204235-plasmid-map-sequence-id-413338

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  22. pCIS-rPALcc

    Plasmid
    #145804
    Purpose
    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells
    Depositor
    Richard Mains, Betty Eipper
    Article
    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed serine 767 to alanine to prevent glycosyl…
    Promoter
    CMV
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    145804-plasmid-map-sequence-id-299336

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  26. pUPD2 AlcR (GB1550)

    Plasmid
    #160585
    Purpose
    Ethanol dependent transcrition factor
    Depositor
    Diego Orzaez
    Insert
    AlcR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160585-plasmid-map-sequence-id-322081

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  32. CALCR-bio-His

    Plasmid
    #53412
    Purpose
    Expresses full-length Calcitonin receptor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CALCR (CALCR Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53412-plasmid-map-sequence-id-98757

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  33. pAc5.1B-lambdaN-HA-DmBicaudalC_P

    Plasmid
    #147163
    Purpose
    Insect Expression of DmBicaudalC
    Depositor
    Elisa Izaurralde
    Insert
    DmBicaudalC (BicC Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    12 silent and three non silent mutattions I331T, …
    Promoter
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147163-plasmid-map-sequence-id-355687

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  37. pAc5.1B-EGFP-DmBicaudalC_P

    Plasmid
    #147158
    Purpose
    Insect Expression of DmBicaudalC
    Depositor
    Elisa Izaurralde
    Insert
    DmBicaudalC (BicC Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    12 silent and three non silent mutattions I331T, …
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147158-plasmid-map-sequence-id-289605

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  40. pUPD2 AlcA (GB1549)

    Plasmid
    #160584
    Purpose
    Ethanol inducible operator
    Depositor
    Diego Orzaez
    Insert
    AlcA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160584-plasmid-map-sequence-id-322082

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Showing: 1 - 40 of 2817 results