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Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH2.30_Gag-MCP-dpol-T2A-eGFP
Plasmid#205538PurposeExpress HIV Gag-MCP-dpol-T2A-eGFPDepositorInsertHIV Gag-MCP-dpol
UseTagsExpressionMammalianMutationpol frameshift sequence recodedPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δgag/pol
Plasmid#101343PurposeDeletion from the start of gag until 229 bases before the cPPTDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta GagPolPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.17_GagZip-MCP-dpol-P2A-eGFP
Plasmid#205532PurposeExpress HIV GagZip-MCP-dpol-P2A-eGFPDepositorInsertGag-MCP-dpol
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, pol fr…PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv
Plasmid#217437PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA E45A
Plasmid#217438PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA E45APromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA M66I
Plasmid#217440PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA M66IPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA Q63/67A
Plasmid#217439PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA Q63A,Q67APromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA Q67H/N74D
Plasmid#217441PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA Q67H,N74DPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralTagsExpressionMutationPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
gB gag LV
Plasmid#12328DepositorInsertgag/pol
UseLentiviralTagsExpressionMammalianMutationThe CypA binding region of the WT gag was replace…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
gS gag LV
Plasmid#12327DepositorInsertgag/pol
UseLentiviralTagsExpressionMammalianMutationThe CypA binding region of the WT gag was replace…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
BIC-Gag-DDX3
Plasmid#233687PurposeExpresses a fusion between the Murine Leukemia Virus Gag polyprotein and the human DDX3 protein to produce virus-like particles loaded with DDX3 and deliver it to target cells.DepositorInsertDEAD-box helicase 3 (DDX3X Human)
UseRetroviralTagsGag (Murine Leukemia Virus)ExpressionMammalianMutationPromoterhCMVAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8.9 NdeltaSB
Plasmid#132929PurposeMinimal HIV-1 packaging plasmid for gag and pol expressionDepositorInsertgag and pol
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
dRT-pMDLg/pRRE
Plasmid#60488Purposegag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)DepositorInsertHIV-1 gag, HIV-1 pol with inactive reverse transcriptase
UseTagsExpressionMutationchanged Asp at position 249 + 250 to ValPromoterAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMVR8.74
Plasmid#22036Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)DepositorInsertgag pol tat rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pfNL43-dGPE-EGFP
Plasmid#36866DepositorInsertNL43-dGPE-EGFP
UseLentiviral and RetroviralTagsExpressionMammalianMutationparts of Gag and Pol were deleted and EGFP insert…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
HDM-Hgpm2
Plasmid#204152PurposeLentivirus helper plasmid coding for Gag/PolDepositorInsertHIV-1 gag/pol
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV delta R8.2
Plasmid#12263PurposeFirst-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.DepositorInsertHIV-1 GAG/POL, Tat and Rev
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
Plasmid#126552PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsCD8a and GFPExpressionMutationDelta gag/polPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 N
Plasmid#132941PurposeMLV packaging plasmid for N-tropic MLV gag-pol expressionDepositorInsertN-tropic MLV gag-pol
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 B
Plasmid#132942PurposeMLV packaging plasmid for B-tropic MLV gag-pol expressionDepositorInsertB-tropic MLV gag-pol
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p8.9 NdeltaSB P90A
Plasmid#132930Purposep8.9 NdeltaSB bearing P90A mutant capsid sequenceDepositorInsertgag P90A and pol
UseLentiviralTagsExpressionMutationP90APromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p8.9 NdeltaSB D185K/D186L
Plasmid#132931Purposep8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequenceDepositorInsertgag and pol D185K/D186L
UseLentiviralTagsExpressionMutationD185K/D186LPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH*DDD
Plasmid#17531DepositorInsertHIV-1 Gag/Pol, Tat, Rev
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_6_002
Plasmid#167348PurposeddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targetsDepositorInsertpol_gag_tat
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA Q67H/N74D
Plasmid#217442PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA Q67H,N74DPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNHP
Plasmid#22500DepositorInsertgag, pol, tat, rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSIV-D3psi/delta env/delta Vif/delta Vpr
Plasmid#132928PurposeProduction of SIV-VLPs containing Vpx proteinDepositorInsertGag, pol, and vpx
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_1_001
Plasmid#167347PurposeddPCR gating control for droplets containing all 5 targets (2 in pol)DepositorInsertpol_gag_tat_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH*deltavpu/RT-
Plasmid#136985PurposeSecond-generation lentiviral packaging plasmid lacking reverse transcriptase activityDepositorInsertHIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
UseTagsExpressionMammalianMutationcarries D110E mutation in the reverse transcripta…PromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CTE-Luc
Plasmid#60881PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTEDepositorInsertLuc-CTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Hgpm2
Plasmid#164441PurposeEncodes codon-optimized HIV gagpol for pseudovirus productionDepositorInsertCodon Optimized HIV-1 GagPol
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only