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We narrowed to 10,753 results for: ESP

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Showing: 1 - 50 of 10753 results

  13. Nesprin3-C-18

    Plasmid
    #58194
    Purpose
    Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    SNAP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  18. pMAT_M5_YESP

    Plasmid
    #197085
    Purpose
    This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif.
    Expression
    Mammalian
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197085-plasmid-map-sequence-id-387437

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  20. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid
    #223509
    Purpose
    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Promoter
    CMV, SP6 and U6
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223509-plasmid-map-sequence-id-448627

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  22. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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  26. mEmerald-Nesprin3-C-18

    Plasmid
    #54203
    Purpose
    Localization: Nuclear Membrane , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54203-plasmid-map-sequence-id-247990

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  27. eSpCas9(1.1)

    Plasmid
    #138555
    Purpose
    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    eSpCas9(1.1)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138555-plasmid-map-sequence-id-274761

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  28. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

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  31. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  33. Dendra2-CathespinB-6

    Plasmid
    #57703
    Purpose
    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. mEmerald-Espin-C

    Plasmid
    #54084
    Purpose
    Localization: Actin Bundling , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Espin (ESPN Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Larry Gerace
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175136-plasmid-map-sequence-id-352023

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  36. pIRESpuro2 AXL

    Plasmid
    #65627
    Purpose
    Expresses AXL on cistronic transcript with puro resistance in mammalian cells
    Depositor
    Aaron Meyer
    Article
    Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.
    Insert
    Tyrosine-protein kinase receptor UFO (AXL Human)
    Expression
    Mammalian
    Mutation
    Spurious PDPRPHR on C terminus
    Promoter
    CMV
    Available Since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65627-plasmid-map-sequence-id-115157

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  37. mTurquoise-Nesprin3-C-18

    Plasmid
    #55572
    Purpose
    Localization: Nuclear Membrane , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  40. mCherry-CathespinB-6

    Plasmid
    #55007
    Purpose
    Localization: Lysosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. mPlum-CathespinB-6

    Plasmid
    #55955
    Purpose
    Localization: Lysosomes, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    CathespinB (Ctsb Rat)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55955-plasmid-map-sequence-id-173178

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  49. mApple-CathespinB-6

    Plasmid
    #54871
    Purpose
    Localization: Lysosomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 50 of 10753 results