Addgene

We narrowed to 17,292 results for: Eomes

Showing: 1 - 50 of 17292 results

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Ii-Str_neomycin

Plasmid

#65308

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Tags

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoMyc-LATS2

Plasmid

#66852

Purpose

Express Myc-tagged LATS2 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS2 (LATS2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119B

Plasmid

#128333

Purpose

Mammalian expression vector of Myc-tagged human UNC119B

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119B (UNC119B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Aa

Plasmid

#128331

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform a

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Ab

Plasmid

#128332

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform b

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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Str-Ii_neomycin

Plasmid

#65312

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Tags

Expression

Mammalian

Mutation

Streptavidin in its cytoplasmid part

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Expression

Mutation

Promoter

CMV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS1 (LATS1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc' CST-1

Plasmid

#66859

Purpose

Express Myc-tagged C.elegans CST-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

CST-1 (cst-1 Nematode)

Use

Tags

Myc

Expression

Mammalian

Mutation

S48N, H50R, S257P and V340A mutations in CST-1 co…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
Eomes gRNA#1

Plasmid

#170834

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Eomes gRNA#3

Plasmid

#170836

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc'-cYAP-1

Plasmid

#66854

Purpose

Express Myc-tagged C.elegans YAP-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoMyc'-EGL-44

Plasmid

#66855

Purpose

Express Myc-tagged C.elegans EGL-44 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

EGL-44 (CELE_F28B12.2 Nematode)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCIneoMyc rat SAPAP1

Plasmid

#40215

Depositor

Yoshimi Takai, Yutaka Hata

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP1 (Dlgap1 Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pClneoMyc human NDR1

Plasmid

#37023

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

NDR1 (STK38 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Eomes

Plasmid

#70271

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Eomes

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Eomes (Eomes Mouse)

Use

Tags

Expression

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Use

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc mouse LNX2

Plasmid

#37010

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

LNX2 (Lnx2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
pClneoMyc human ERBIN

Plasmid

#40214

Depositor

Yutaka Hata

Article

Ohno et al Oncogene. 2002 Oct 10;21(46):7042-9.

Insert

ERBIN (ERBIN Human)

Use

Tags

myc

Expression

Mammalian

Mutation

L143S, S274L and P338L mutation in ERBIN compared…

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-H1-GFP-neomycin

Plasmid

#37632

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-neomycin-PC-MYC

Plasmid

#184550

Purpose

Retroviral vector to express human PC with MYC tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Eomes-3xT7

Plasmid

#200888

Purpose

Inducible lentiviral expression of Eomes

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Eomes (Eomes Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc mouse LNX1

Plasmid

#37009

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

LNX1 (Lnx1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
pClneoMyc human MST2

Plasmid

#37022

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MST2 (STK3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pClneoMyc human CIN85

Plasmid

#47935

Purpose

expresses Myc tagged human CIN85 in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

CIN85 (SH3KBP1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 2-665 of human CIN85; D114G

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc human MOB1

Plasmid

#37024

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MOB1B (MOB1B Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Eomes

Plasmid

#128825

Purpose

dox-dependent expression of mouse Eomes

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Eomes (Eomes Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Shorter isoform

Promoter

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoMyc mouse Numb

Plasmid

#41712

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

Numb (Numb Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 8-853

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T74A

Plasmid

#47939

Purpose

expresses Myc tagged human MOB1 containing T74A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T74A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T12A

Plasmid

#47936

Purpose

expresses Myc tagged human MOB1 containing T12A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T12A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T35A

Plasmid

#47937

Purpose

expresses Myc tagged human MOB1 containing T35A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pClneoMyc rat S-SCAM alpha

Plasmid

#40213

Depositor

Yoshimi Takai, Yutaka Hata

Article

Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

Insert

S-SCAM (Magi2 Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
PiggyBac-TetO-Ascl1-neomycin

Plasmid

#176482

Purpose

TetO-Ascl1 in a PiggyBac vector with neomycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Ascl1 (Ascl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc MOB1 T12/35A

Plasmid

#47938

Purpose

expresses Myc tagged human MOB1 containing T12A and T35A mutations

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T12A and T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-EOMESwt-IDR

Plasmid

#145231

Purpose

Expresses fusion of GAL4 DNA-binding domain and EOMESwt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

EOMESwt-IDR (EOMES Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-fSNAP-Phleomycin

Plasmid

#188915

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-EOMES-Adel-IDR

Plasmid

#145230

Purpose

Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

EOMES-Adel-IDR (EOMES Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

Plasmid

#223959

Purpose

Dual fluorescent reporter for histone H2B and for APC/C activity

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGE144 (MutaEco; aTC-induced)

Plasmid

#189824

Purpose

Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

Depositor

Seokhee Kim

Article

Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

Insert

EcoRNAPα-PmCDA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

tetR/tetA promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDae069 (eMutaT7[PmCDA1] with optimized RBS)

Plasmid

#187621

Purpose

Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

PmCDA1-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDae029 (eMutaT7[TadA-8e])

Plasmid

#187620

Purpose

Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

TadA-8e-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a(+)-DR RecR

Plasmid

#12617

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a(+) SurE D9A

Plasmid

#12686

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE

Use

Tags

His

Expression

Bacterial

Mutation

changed Aspartate 9 to Alanine

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pET-21a(+) SurE

Plasmid

#12669

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE (TM1662 T.maritima)

Use

Tags

His

Expression

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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