Skip to main content
Addgene
Showing: 1 - 50 of 1697 results

  1. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid
    #123350
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-Cameleon
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123350-plasmid-map-sequence-id-307103

    Enlarge

  3. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123329
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-AKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123329-plasmid-map-sequence-id-313436

    Enlarge

  4. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid
    #123330
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-AKAR(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123330-plasmid-map-sequence-id-306911

    Enlarge

  6. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid
    #123339
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123339-plasmid-map-sequence-id-307068

    Enlarge

  7. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid
    #123343
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-CKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123343-plasmid-map-sequence-id-307094

    Enlarge

  10. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid
    #123340
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123340-plasmid-map-sequence-id-307135

    Enlarge

  11. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

    Plasmid
    #123344
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-CKAR(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123344-plasmid-map-sequence-id-307096

    Enlarge

  12. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid
    #182395
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    Use
    Tags
    Vesicle Nucleating peptide (VNp) & Leucine Zi…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182395-plasmid-map-sequence-id-364922

    Enlarge

  13. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123338
    Purpose
    Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    DAKAP-Venus-cpVenus-FLARE-AKAR
    Use
    Tags
    N-terminal 30 amino acids from DAKAP1, Venus, and…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123338-plasmid-map-sequence-id-307174

    Enlarge

  14. pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123337
    Purpose
    Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Lyn-Venus-cpVenus-FLARE-AKAR
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, Ve…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123337-plasmid-map-sequence-id-313437

    Enlarge

  25. pSLIK-Venus

    Plasmid
    #25734
    Purpose
    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25734-plasmid-map-sequence-id-12059

    Enlarge

  30. pCAGGs-Venus

    Plasmid
    #127346
    Purpose
    Expresses Venus constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Venus Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    127346-plasmid-map-sequence-id-246571

    Enlarge

  35. lentiCas9-Venus

    Plasmid
    #70267
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    Cas9
    Venus Reporter
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS and P2A
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70267-plasmid-map-sequence-id-126420

    Enlarge

  38. pENTR-Venus

    Plasmid
    #139551
    Purpose
    Venus ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139551-plasmid-map-sequence-id-274619

    Enlarge

  45. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

    Enlarge

Showing: 1 - 50 of 1697 results