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Plasmid#41812DepositorInsertD10 Super-2 (IL2 Human)
UseTags8x HisExpressionInsectMutationPromoterPolyhedronAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDD108
Plasmid#119884PurposeIntegration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas9D10A_GFP
Plasmid#44720PurposeCo-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9D10A-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationD10A mutation- nickase mutantPromoterCAGAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm)
Plasmid#48886PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA
Plasmid#48884PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDD103
Plasmid#119887PurposeConstitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_MFSD10
Plasmid#131890PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertMFSD10 (MFSD10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pID1072
Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD102
Plasmid#131403PurposeE. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD102
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LD101
Plasmid#69160PurposeIntegration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg1-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm) -R
Plasmid#48888PurposeNegative feedback plasmid from oscillator without luxRDepositorInsertaiiA
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pABD1031
Plasmid#68004PurposeProtein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in plantaDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
UseTagseYFPExpressionPlantMutationPromoter2X Cauliflower Mosaic Virus 35S promoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHCHD10_Halo_N_allele
Plasmid#178118PurposeDonor vector for endogenous tagging of human CHCHD10 at the N-terminus with halotagInsertHalotag (CHCHD10 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LD102
Plasmid#69161PurposeIntegration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg2-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CA_p.D1045V
Plasmid#81702PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CA (PIK3CA Human)
UseGateway entry vectorTagsExpressionMutationD1045VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD10_46
Plasmid#1432DepositorTypeEmpty backboneUseTagsOriF1<<, OriF1>>, lacZN, unc54N, unc5…ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only