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We narrowed to 2,481 results for: D10

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Showing: 1 - 20 of 2481 results

  1. PROM_10

    Plasmid
    #201301
    Purpose
    D10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D10: Promoter of TraesCS3D02G517600
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201301-plasmid-map-sequence-id-410981

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  2. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Publication
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9_D10A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41816-plasmid-map-sequence-id-116715

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  3. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Publication
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44720-plasmid-map-sequence-id-179979

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  4. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42335-plasmid-map-sequence-id-275689

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  5. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63593-plasmid-map-sequence-id-181681

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  9. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42333-plasmid-map-sequence-id-59273

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  11. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60367-plasmid-map-sequence-id-456413

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  17. pX330A_D10A-1x2

    Plasmid
    #58772
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58772-plasmid-map-sequence-id-91082

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  19. 2P65

    Plasmid
    #60113
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv089580:MAC01Q-D10:C26843
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
Showing: 1 - 20 of 2481 results