1,878 results

1. pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

   Plasmid

   #24033

   Depositor

   Cori Bargmann

   Article

   Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

   Insert

   Npr1 (npr-1 Nematode)
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a Ce-iPGM-bio

   Plasmid

   #162559

   Purpose

   Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   C. elegans iPGM bio-6His (ipgm-1 Nematode)
   

   Tags

   6His tag, T7 tag, Thrombin cleavage site, and bio…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH2685

   Plasmid

   #191372

   Purpose

   Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   Tetanus Toxin Light Chain
   

   Tags

   wCherry

   Expression

   Worm

   Promoter

   Punc-4

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLT 653

   Plasmid

   #59996

   Purpose

   Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

   Depositor

   Lisa Timmons

   Article

   Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

   Inserts

   dhc-3 (dhc-3 Nematode)
   klp-16 (klp-16 Nematode)
   

   Expression

   Bacterial

   Mutation

   partial genomic

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSDH54

   Plasmid

   #139052

   Purpose

   MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   tbb-2 3'UTR (tbb-2 Nematode)
   

   Expression

   Worm

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNM3774

   Plasmid

   #154117

   Purpose

   tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL44 - Phsp16.48::MosTase::glh-2utr

   Plasmid

   #19333

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Phsp-16::Mos1 transposase
   

   Expression

   Worm

   Available Since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOD1988-epiDEG

   Plasmid

   #89357

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Promoter

   Pdpy-7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lis-1::L4440

   Plasmid

   #11336

   Depositor

   Guy Caldwell

   Insert

   lis-1 (lis-1 Nematode)
   

   Use

   RNAi

   Expression

   Worm

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pGLOW31

    Plasmid

    #172698

    Purpose

    C. elegans mScarlet co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mScarlet
    

    Tags

    mScarlet

    Expression

    Worm

    Promoter

    myo-3

    Available Since

    Aug. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pJW2086

    Plasmid

    #154335

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJS012

    Plasmid

    #188335

    Purpose

    PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::mTagBFP2::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

    Plasmid

    #19326

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::twk-18(cn110)cDNA
    

    Expression

    Worm

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS178

    Plasmid

    #215678

    Purpose

    Empty repair plasmid for ChrII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxP + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD285

    Plasmid

    #66826

    Purpose

    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mKate2

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pSEM272 - MCS mosTI Pmlc-2_GFP target

    Plasmid

    #159834

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI Pmlc-2_GFP target
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCZGY405

    Plasmid

    #172694

    Purpose

    Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    rab-3 (rab-3 Nematode)
    

    Tags

    Punc-25 and mcherry

    Expression

    Worm

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOD2046-bwmDEG

    Plasmid

    #89367

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Promoter

    Pmyo-3

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW6.01

    Plasmid

    #62882

    Purpose

    Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    GFP::MEG-3
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    meg-3

    Available Since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge