1,904 results

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pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

Plasmid

#24033

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

Npr1 (npr-1 Nematode)

Use

Cre/Lox

Tags

GFP

Expression

Worm

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pDD315 (mTurquoise2^SEC^2xHA)

Plasmid

#73343

Purpose

mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

Insert

mTurquoise2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans codon-optimized mTurquoise2

Expression

Worm

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET_MBP-RGG-GFP-RGG

Plasmid

#124949

Purpose

Expression of MBP-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP, His, and MBP

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJW2086

Plasmid

#154335

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSH83

Plasmid

#212989

Purpose

expresses miniSOG in C. elegans

Depositor

David M. Miller

Article

Cuentas-Condori et al Elife. 2019 Oct 4;8. pii: 47918. doi: 10

Insert

flp-13 promoter (flp-13 Nematode)

Expression

Worm

Promoter

flp-13

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDD285

Plasmid

#66826

Purpose

mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mKate2

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
pCFJ108 - pENTR221 - [cbr-unc-119(+)]

Plasmid

#200367

Purpose

cbr-unc-119 rescue gene for use as co-injection marker.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[cbr-unc-119(+)]

Expression

Worm

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJRK248

Plasmid

#173755

Purpose

co-injection plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR

Expression

Worm

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159873

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM246 - MCS mosTI unc-119 target

Plasmid

#159821

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI unc-119 target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

Plasmid

#19326

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::twk-18(cn110)cDNA

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pNM3755

Plasmid

#154116

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::act-4 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJL049

Plasmid

#198809

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR

Expression

Worm

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

Plasmid

#193048

Purpose

Heatshock expressed Cas9

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR

Use

CRISPR

Expression

Worm

Promoter

hsp16.41p

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDD373

Plasmid

#137021

Purpose

SapTrap GFP-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized GFP

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized GFP

Expression

Worm

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJRK246

Plasmid

#173741

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

wrt-2 promoter

Use

Other

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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