-
Plasmid#163915PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-ProSgH
Plasmid#163974PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG
Plasmid#158145PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA
Plasmid#134673PurposeExpresses His6-TglA in E. ColiDepositorInsertTglA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSETB BS2mT4L1
Plasmid#141203PurposeChimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.DepositorInsertBS2m-T4L1
UseTagsExpressionBacterialMutationE11QPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ECM_unstable
Plasmid#127607Purposehigh-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3DepositorInsertUnstable ECM33 intron (Mut)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ECM33_intron
Plasmid#127596Purposelow-copy URA-selectable plasmid, ECM33 intron inserted into URA3DepositorInsertECM33 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-RPL27B_intron
Plasmid#127604Purposelow-copy URA-selectable plasmid, RPL27B intron inserted into URA3DepositorInsertRPL27B intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-APS3_intron
Plasmid#127605Purposelow-copy URA-selectable plasmid, APS3 intron inserted into URA3DepositorInsertAPS3 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HNT2_intron
Plasmid#127603Purposelow-copy URA-selectable plasmid, HNT2 intron inserted into URA3DepositorInsertHNT2 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(NT)
Plasmid#138258PurposeExpression of a non-targeting sgRNA to be used as a control in a yeast dual reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS414-ATG41
Plasmid#113080PurposeContain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.DepositorInsertATG41
UseTagsExpressionBacterial and YeastMutationPromoterATG41 endogenous promoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-50
Plasmid#111315PurposepRS314 snu114-50DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114-50 (C928R)PromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-Prp28
Plasmid#111285PurposePrp28 expression plasmidDepositorInsertPRP28
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315 U2 IIc+
Plasmid#111357PurposeU2 snRNA with IIc+ mutation on a pRS315 plasmidDepositorInsertsnR20
UseTagsExpressionYeastMutationstem II IIc+ mutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
sub2-5 pRS315
Plasmid#111302Purposesub2-5 in pRS315DepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-5PromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-15
Plasmid#111312PurposepRS314 snu114-15DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114-15 (K146I)PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-14
Plasmid#111311Purposesnu114-14DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114-14PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303 prp8-1
Plasmid#111306Purposeprp8-1DepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationprp8-1 ( G1801D )PromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-40
Plasmid#111314Purposesnu114-40DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114-40 (M842R)PromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-30
Plasmid#111313Purposesnu114-30DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114-30 (G646R)PromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 snu114-60
Plasmid#111316PurposepRS314 snu114-60DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114-60 (K939stop)PromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314 HA-SNU114
Plasmid#111318PurposeHA-SNU114DepositorInsertSNU114
UseTagsHAExpressionYeastMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2C pRSETB
Plasmid#104500Purposefluorescent reporter for calcium signalingDepositorInsertTwitch-2C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ZmSCE1b-Myc
Plasmid#91930PurposeBacterial expression of maize SCE1bDepositorInsertZmSCE1b-Myc
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro3GFP
Plasmid#82367PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro3GFP
UseTags6xHISExpressionBacterialMutationC48S/Q80R/N149C/S202CPromoterT7Available sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v2
Plasmid#79538PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 2DepositorInsertAmrose v2
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.9
Plasmid#78179PurposeExpresses-rsGreen0.9 in E. coli strains.DepositorInsertrsGreen0.9
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.7b
Plasmid#78178PurposeExpresses rsGreen0.7b in E. coli strains.DepositorInsertrsGreen0.7b
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa
Plasmid#78183PurposeExpresses pcDronpa in E. coli strainsDepositorInsertpcDronpa
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaNdeltaC
Plasmid#32030DepositorInsertDCR1(107-355)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1delta2d
Plasmid#32028DepositorInsertDCR1(15-260)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS306Sup35R1+8H1Q
Plasmid#17722DepositorInsertSup35R1+8H1Q (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII282Pglu-4m
Plasmid#13564DepositorInsertFLIPglu F16A T282S-ECFP-N283R
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS415-IMP2'
Plasmid#10992DepositorInsertIMP2' (IMP2' Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SSM1
Plasmid#11003DepositorInsertSSM1 (SDT1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-1
Plasmid#89972Purposeprp16-1 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-1 allele (Y386D)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-3 in pRS314
Plasmid#90454PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-3 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-4 in pRS314
Plasmid#90455PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-4 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-6 in pRS314
Plasmid#90456PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-6 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413-GAL1-yEGFP1
Plasmid#40235DepositorInsertyEGFP1
UseTagsHIS3MX6ExpressionBacterialMutationPromoterGAL1Available sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-mNeongreen
Plasmid#182388PurposeBacterial expression of Vesicle Nucleating peptide6-mNeongreen fusionDepositorInsertVNp6-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mMaple3-V157I
Plasmid#98577Purposephotoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmMaple3-V157I
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-mNeongreen
Plasmid#182389PurposeBacterial expression of Vesicle Nucleating peptide15-mNeongreen fusionDepositorInsertVNp15-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen
Plasmid#182387PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen fusionDepositorInsertVNp-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SuperNova Green/ pRSETB
Plasmid#124839Purposebacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent proteinDepositorInsertSuperNova Green
UseTagsNo tagExpressionBacterialMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-COF1
Plasmid#31844DepositorInsertCofilin (COF1 Budding Yeast)
UseTagsGFP (S65T)ExpressionYeastMutationPromoterT7Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only