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Plasmid#177901PurposeBacterial expression of his10-tagged human cd96 Intracellular DomainDepositorInsertCD96 (aa 557-585 only) (CD96 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMBP and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4
Plasmid#226372PurposeProduction of recombinant mEGFP-D4, a fluorescent probe binding cholesterolDepositorInsertD4 (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-zDjCas13d-His
Plasmid#234481PurposePlasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)DepositorInsertDjCas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-EGFP-CNA35
Plasmid#61603Purposebacterial expression of N-terminal fusion protein of CNA35 with EGFPDepositorInsertEGFP and CNA35
UseTagsHis tagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-CNA35
Plasmid#61607Purposebacterial expression of N-terminal fusion protein of CNA35 with mCherryDepositorInsertmCherry and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-2xdBroccoli
Plasmid#66843PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
UseTagsHis6 tag and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-uvsY
Plasmid#163914PurposeExpresses uvsY for bacterial expression and affinity purificationDepositorInsertuvsY (uvsY Enterobacteria phage T4)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniE-DBCO
Plasmid#181985PurposeUsed to label proteins with the MiniR genetically encoded tagDepositorInsertMiniE-DBCO
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
hPU.1ΔN165-pET28b
Plasmid#199796PurposeExpressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coliDepositorInsertspi1 (SPI1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-EsCas13d
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorArticleInsertHalotag-CNA35
UseTagsHalotagExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a/Cas9-Cys
Plasmid#53261PurposeExpresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal CysteineDepositorInsertCas9-Cys
UseTags6X His tag, HA epitope, and NLSExpressionBacterialMutationAdditional Cysteine is added to the C-terminal re…PromoterT7 PromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpRY-His
Plasmid#179320PurposePlasmid for bacterial expression and purification of SpRY-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpRY
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…PromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTurquoise2-CNA35
Plasmid#61602Purposebacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2DepositorInsertmTurqouise and CNA35
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Keap1
Plasmid#62454PurposeHis6 tagged Keap1 for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspWYL1
Plasmid#108306PurposeExpresses E. coli codon optimized RspWYL1 in the pET28a backbone.DepositorInsertRspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…PromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mAmetrine-CNA35
Plasmid#61604Purposebacterial expression of N-terminal fusion protein of CNA35 with mAmetrineDepositorInsertmAmetrine and CNA35
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmOrange-CNA35
Plasmid#61605Purposebacterial expression of N-terminal fusion protein of CNA35 with LSSmOrangeDepositorInsertLSSmOrange and CNA35
UseTagsHis tagExpressionMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d
Plasmid#108305PurposeExpresses E. coli codon optimized RspCas13d in the pET28a backbone.DepositorInsertRspCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-FnCas12a-TEV
Plasmid#174658PurposeFnCas12a expression plasmidDepositorInsertFnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT
Plasmid#173943PurposeBacterial expression of isolated PARP1 CAT domainDepositorInsertPARP1-CAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-DogTag
Plasmid#105629PurposePlasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJrDepositorInsertSUMO-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
UseTags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-Broccoli
Plasmid#66788PurposeExpresses Broccoli in the F30 scaffold in bacterial cellsDepositorInsertBroccoli in the F30 scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hUba2-His
Plasmid#53117Purposeexpression of full length human Uba2 with Cterminal His-tag in E.coliDepositorInsertUBA2 (UBA2 Human)
UseTagsHis and T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Tau352
Plasmid#100092PurposeExpresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tagDepositorInsertFetal microtubule-associated protein tau (MAPT Human)
UseTagsHis6ExpressionBacterialMutationwild type sequencePromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL18
Plasmid#183390PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
UseTagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…PromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HisYFP-Sp100
Plasmid#53141PurposeE.coli expression of HisYFP tagged human Sp100 fragmentDepositorInsertSp100A fragment (SP100 Human)
UseTagsHis-YFPExpressionBacterialMutationfragment (amino acids 71-480); deletion of L75PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL1B
Plasmid#183386PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL1B (Il1b Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
6his-WT gusA-pET28a+
Plasmid#61156PurposeN -terminal His tagged wild-type GUS expression vectorDepositorInsertgusA
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-MED1LCD
Plasmid#204404PurposeE. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tagDepositorInsertMED1(947-1581) (MED1 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-MjDim1
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
UseTags6x His tag, FLAG tag, SUMO tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
UseTagsHA tag and Histidine tagExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-MinD
Plasmid#133622PurposeExpression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.DepositorInsertEGFP-MinD (minD E. coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only