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We narrowed to 216 results for: Bioid

Showing: 181 - 200 of 216 results
  1. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

    Plasmid
    #218833
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.
    Depositor
    Insert
    TLK1 Q479E (TLK1 Human)
    Use
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Q479E
    Promoter
    CMV
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

    Plasmid
    #218834
    Purpose
    Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.
    Depositor
    Insert
    TLK1 (TLK1 Human)
    Use
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    BirA(R118G)-HA
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    Available since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
  4. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid
    #118375
    Purpose
    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.
    Depositor
    Type
    Empty backbone
    Use
    Destination
    Tags
    HA-BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pKK-TEV-BirA-FLAG

    Plasmid
    #105786
    Purpose
    Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-BirA-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pKK-FLAG-BirA-TEV

    Plasmid
    #105770
    Purpose
    Expression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    FLAG-BirA-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 216 results