We narrowed to 856 results for: Hr;

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KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

Plasmid

#124175

Purpose

piggyBac-based Dox-inducible expression (very weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

Plasmid

#124174

Purpose

piggyBac-based Dox-inducible expression (weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

Plasmid

#192828

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBA1015

Plasmid

#189589

Purpose

T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

T7gp1.7 Locus 250bp Homology Arms

Use

Synthetic Biology

Expression

Bacterial

Mutation

Encodes for a full deletion of T7gp1.7

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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KA2058_pX330A-G2P

Plasmid

#124202

Purpose

Expression of SpCas9 and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60-Ptb

Plasmid

#38245

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Ptb (Ptbp1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK-3xFLAG

Plasmid

#163452

Purpose

lentiviral overexpression vector for human NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmCherry

Plasmid

#192827

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VRER

Plasmid

#159984

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VRER

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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KA0718_pPB-TREtight-cHA-IRESVenus

Plasmid

#124171

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-T-V5-GR-2A-H2BmCherry-IP

Plasmid

#171500

Purpose

Overexpression of T(Brachyury)-GR

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFBD-ESCRT 0

Plasmid

#21499

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)

Tags

GST and His-MBP

Expression

Insect

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pMP4518

Plasmid

#107778

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pBBR1-mcs5

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V174C

Plasmid

#205078

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V174C

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

Plasmid

#140221

Purpose

Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1 (Cas12a) N terminal half (406) with zipper

Expression

Mammalian

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBA1032

Plasmid

#189580

Purpose

T4soc deletion HR vector 250bp HR Arms (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

T4soc Deletion Locus 250bp Homology Arms

Use

Unspecified

Mutation

Encodes for a full deletion of T4soc

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMP4516

Plasmid

#107775

Purpose

Expression of ECFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-ECFP in pBBR1-mcs5

Tags

pLacZ-ECFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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KA2601_eSpCas9-G2P

Plasmid

#124203

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKCZeta KR HA Tagged

Plasmid

#24608

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Mutation

K281R mutation in the ATP binding domain causing …

Available Since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
KA1065_pPB-hCMV*1-cHA-IRESH2BmCherry

Plasmid

#124170

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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