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Showing: 181 - 200 of 225 results

  2. pDOC-K-glmS

    Plasmid
    #158058
    Purpose
    Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Inserts
    glmS homologous region 1
    glmS homologous region 2 + MCS3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158058-plasmid-map-sequence-id-309430

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  6. pG0A0-1-1-PBAD-dCas9

    Plasmid
    #208815
    Purpose
    Expresses arabinose-inducible dCas9, codon-optimized for Salmonella
    Depositor
    Marc Erhardt
    Article
    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:
    Insert
    Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinsoe-inducible promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    208815-plasmid-map-sequence-id-468502

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  7. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Use
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  8. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211844
    Purpose
    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Insert
    LRR-NEL
    Use
    Synthetic Biology
    Tags
    3xNLS-HA and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    UBIQUITIN10
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    211844-plasmid-map-sequence-id-416933

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  10. pNB22

    Plasmid
    #103059
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    hmpAp
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103059-plasmid-map-sequence-id-199053

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  11. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  14. pNB23

    Plasmid
    #103058
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hydrogen peroxide-inducible promoter katGp
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103058-plasmid-map-sequence-id-199054

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  15. pSUB7

    Plasmid
    #169698
    Purpose
    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169698-plasmid-map-sequence-id-334547

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  16. pSU315

    Plasmid
    #169697
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169697-plasmid-map-sequence-id-334518

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  18. pSU314

    Plasmid
    #169696
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169696-plasmid-map-sequence-id-334524

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  19. pSU312

    Plasmid
    #169694
    Purpose
    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xFLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169694-plasmid-map-sequence-id-334513

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  20. pSU313

    Plasmid
    #169695
    Purpose
    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    Use
    Tags
    1xFLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169695-plasmid-map-sequence-id-334516

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Showing: 181 - 200 of 225 results