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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,100 results for: cas12a

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Showing: 181 - 200 of 1100 results

  1. nLbCas12a

    Plasmid
    #213745
    Purpose
    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nLbCas12a
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213745-plasmid-map-sequence-id-422202

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  3. LbCas12a-niCPE

    Plasmid
    #213747
    Purpose
    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP-nLbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A in LbCas12a
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213747-plasmid-map-sequence-id-422190

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  9. 6xHis-MBP-huErCas12a

    Plasmid
    #174685
    Purpose
    Expresses 6xHis-MBP tagged ErCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huErCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis
    Expression
    Bacterial
    Mutation
    Q926R mutation is observed as indicated from the …
    Promoter
    T7
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    174685-plasmid-map-sequence-id-346198

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  10. 6xHis-MBP-huTsCas12a

    Plasmid
    #174687
    Purpose
    Expresses 6xHis-MBP tagged TsCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huTsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174687-plasmid-map-sequence-id-346204

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  12. pLenti-Cas12a2-EGFP

    Plasmid
    #258653
    Purpose
    Expresses mammalian codon-optimized CRISPR-Cas12a2 nuclease (Sulfuricurvum sp. PC08-66) and EGFP under EFS promoter. 3rd-generation lentiviral backbone.
    Depositor
    Yang Liu
    Publication
    Scholz et al Nature. 2026 May 6. doi: 10.1038/s41586-
    Inserts
    Cas12a2
    EGFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Available since
    July 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    258653-plasmid-map-sequence-id-515050

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  13. pYL-kasOp-FnCas12a2

    Plasmid
    #177874
    Purpose
    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.
    Depositor
    Yunzi Luo
    Publication
    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptomyces codon optimized FnCas12a
    Use
    CRISPR
    Promoter
    kasOp*
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177874-plasmid-map-sequence-id-352015

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  14. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155046-plasmid-map-sequence-id-308670

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  15. pTE3987

    Plasmid
    #137716
    Purpose
    Expresses dLbCas12a-RVRR-BE in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-RVRR-BE
    Tags
    6xHis, APOBEC-1, SV40 NLS, and UGI
    Expression
    Mammalian
    Mutation
    G532A, K538V, Y542R, K595R
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137716-plasmid-map-sequence-id-274024

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  17. LbCas12a-2C-NLS in pCSDest

    Plasmid
    #126638
    Purpose
    Expresses LbCas12a-2C-NLS in mammalian cell
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126638-plasmid-map-sequence-id-247967

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  18. LbCas12a-crRNA

    Plasmid
    #213753
    Purpose
    For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-crRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213753-plasmid-map-sequence-id-422200

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Showing: 181 - 200 of 1100 results