We narrowed to 922 results for: cas12a

1. pT7-AsCas12a_crRNA-site4 (MSP3511)

   Plasmid

   #160139

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-opCas12a-Ultra-6xNLS

   Plasmid

   #209024

   Purpose

   Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/M537R/S542R/F870L

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R, R883K, R887A

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2xNLS-NLP-cMyc LbaCas12a

   Plasmid

   #182123

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc LbaCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

   Plasmid

   #160610

   Purpose

   Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:AsCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-AsCas12a_crRNA-site3 (RTW549)

   Plasmid

   #160138

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

   Plasmid

   #160575

   Purpose

   LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:LbCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

   Plasmid

   #244837

   Purpose

   Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   LbCas12a-RVQ
   

   Use

   CRISPR

   Tags

   2xSV40 NLS

   Expression

   Mammalian

   Mutation

   G146R/R182V/E795Q

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS

   Plasmid

   #244840

   Purpose

   Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Lbm6-Cas12a
   

   Use

   CRISPR

   Tags

   10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   D535G/S551F/D665N

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SM470_EF1A-dEnAsCas12a-T2A-EGFP

    Plasmid

    #244222

    Purpose

    Mammalian expression of dEnAsCas12a with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM6_CL7_Bac_2NLS-RVQ (LbCas12a)-2NLS

    Plasmid

    #244841

    Purpose

    Bacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 2xSV40 NLS

    Expression

    Bacterial

    Mutation

    G146R/R182V/E795Q

    Promoter

    T7

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SM468_EF1A-dHyperLbCas12a-T2A-EGFP

    Plasmid

    #244220

    Purpose

    Mammalian expression of dHyperLbCas12a with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SM471_EF1A-dEnAsCas12a-T2A-BlastR

    Plasmid

    #244223

    Purpose

    Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. SM469_EF1A-dHyperLbCas12a-T2A-BlastR

    Plasmid

    #244221

    Purpose

    Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNS49 (enAsCas12a-KRAB piggyBac)

    Plasmid

    #217335

    Purpose

    piggyBac expresion of enAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    enAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-enCas12a-A3A-N57G

    Plasmid

    #193651

    Purpose

    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-N57G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

    Plasmid

    #160574

    Purpose

    AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:AsCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-FnCas12a-TLR-MCV1-gRNA

    Plasmid

    #117412

    Purpose

    FnCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    FnCas12a gRNA targeting TLR 2.0
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AsCas12a-6xNLS-E174R/S542R (pRG232)

    Plasmid

    #149723

    Purpose

    Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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